DEVELOPMENT OF THE GENE-TRAP MICROARRAY AND ITS APPLICATION TO IMMUNOPATHOLOGY

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DNA arrays are capable of profiling the expression patterns of many genes in a single experiment. After finding a gene of interest in a DNA array, however, labor-intensive gene targeting experiments sometimes must be performed for the in vivo analysis of the gene function. With random gene segments are analyzed. In order to combine the benefits of the above two experimental systems, we first created about nine hundred gene-trapped mouse ES cell dones(http://bsw3.aist-nara.ac.jp/kawaichi/naistrap.html)and then constructed arrays of cDNAs derived from the disrupted genes Using these arrays, we identified a novel gene predominantly expressed in the mouse brain, and the corresponding ES cell done was used to produced mice homozygous for the disrupted allele of the gene. Detailed analysis of the knockout mice revealed that the gene trap vector completely abolished gene expression downstream of its integration site. Therefore, identification of a gene or a novel-gene candidate with an interesting expression pattern using this type of DNA arrays immediately allows the production of knockout mice from an ES cell clone with a disrupted allele of the sequence of *rest.