Antifungal susceptibility of new genotype of Candida albicans strains with group 1 intron

1组内含子白色念珠菌新基因型菌株的抗真菌敏感性

• 批准号: 14570231
• 负责人: MIKAMI Yuzuru
• 金额: $ 2.24万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570231/
• 关键词: Candid a albicans; Candida dubliniensis; genotyping; group 1 intron; drug susceptibility; clinical isolates; イントロン; フルシトシン; C.dubliniensis; new genotype E; C. dubliniensis; リボゾーム

The genetic diversity of recent clinical isolates of Candida albicans in Japan and Thailand was studied on the basis of amplified DNA band lengths determined with a specific PCR Primer. Our analyses of 401 clinical isolates of C.albicans showed that they could be classified into five genotypes A, B, C, D (C.dubliniensis) and a new genotype E. Most of the strains from Japan as well as Thailand were genotype A, and C was a minor group. The genotype E was characterized to have a group 1 intron-like sequence, which is longer that hitherto reported ones ad which has a nucleotide sequence length of 962-bp. Our analysis of the 962-bp sequence indicated that it is composed of an intron similar to that of C.dubliniensis of 621-bp with a 341-bp insertion. Analysis of the sequence of ITS region of the E showed that the sequence is identical with those of C.albicans. Throught the study, the possible horizontal transfer of the group 1 intron between C.dubliniensis and C.albicans E was suggested. A high degree of correlation between the presence of group l intron in genotype E and the susceptibility to flucytosine was observed. Five and two strains of C.dubliniensis from Japan and Thailand, respectively. They were not isolates from AIDS patients, and not resistant to azole-type antifungals such as fluconazole. Most of the resistant strains were found to belong to genotype A, B, o r, C, in Japan and Thailand. Detailed mechanisms of resistant of C.albicans (not C.dubliniensis) to azole and flucytosine ant ifungals were analyzed using micro-array method using home made array system in our laboratory.

利用特异PCR引物测定扩增DNA条带长度，研究了日本和泰国近期临床分离的白色念珠菌的遗传多样性。通过对401株临床分离的白色念珠菌的分析，发现它们可分为A、B、C、D (dubliniensis) 5个基因型和一个新的基因型e。大部分来自日本和泰国的菌株为A基因型，C基因型占少数。基因型E的特征是具有1组内含子样序列，该序列比迄今报道的更长，核苷酸序列长度为962 bp。对962-bp序列的分析表明，该序列由一个内含子组成，与C.dubliniensis的621-bp相似，插入341-bp。对E的ITS区序列分析表明，该序列与白色念珠菌的序列相同。通过研究，提示1族内含子可能在都柏林梭菌和白色梭菌E之间水平转移。观察到基因型E中1族内含子的存在与氟胞嘧啶易感性之间存在高度相关。分别来自日本和泰国的5株和2株dubliniensis。它们不是从艾滋病患者身上分离出来的，对氟康唑等唑类抗真菌药物没有耐药性。在日本和泰国发现的耐药菌株多为A、B、o、C基因型。利用自制的阵列系统，采用微阵列法分析了白色念珠菌（非dubliniensis）对唑类和氟胞嘧啶类蚂蚁的耐药机制。

项目成果

Myoken Y, Mikami Y et al.: "A tropical antifungal medication in immunocompromised patients"Oral Surgery Oral Medicine Oral Pathol. 381-380 (2004)

Myoken Y、Mikami Y 等人：“免疫功能低下患者的热带抗真菌药物”口腔外科口腔医学口腔病理。

Melkusova S, Mikami Y et al.: "The efficiency of benzothiazole ABP, echinocandin, micafungin, and amphotericin B ma fiuconazle -resistant Candida albicans and Candida dubliniensis"Pharmzie. (in press). (2004)

Melkusova S、Mikami Y 等人：“苯并噻唑 ABP、棘白菌素、米卡芬净和两性霉素 B 对氟康唑耐药的白色念珠菌和都柏林念珠菌的功效”Pharmzie。

Myoken Y, Mikamu Y et al.: "Breakthrough fungemia caused by azole-resistant Candida albicans in neutropenic patients with acute leukemia"J.Oral.Pathol.Med.. 32. 1496-1497 (2003)

Myoken Y、Mikamu Y 等人：“在患有急性白血病的中性粒细胞减少症患者中由唑类耐药白色念珠菌引起的突破性真菌血症”J.Oral.Pathol.Med.. 32. 1496-1497 (2003)

Katsu M, Mikami Y, et al.: "Immunomangnetic isolation of Cryptococcus neoformans by beads coated with anti-Cryptococcus serum"Jpn.J.Med.Mycol.. 44. 139-144 (2003)

Katsu M, Mikami Y, et al.：“通过涂有抗隐球菌血清的珠子对新型隐球菌进行免疫磁性分离”Jpn.J.Med.Mycol.. 44. 139-144 (2003)

Myoken Y, Mikami Y, et al.: "Breakthrough fungemia caused by azole-resistant Candia albicans in neutropenic patients with acute"Clin.Infect.Dis.. 36. 1496-1497 (2003)

Myoken Y、Mikami Y 等人：“急性中性粒细胞减少症患者中由唑类抗性白色念珠菌引起的突破性真菌血症”Clin.Infect.Dis.. 36. 1496-1497 (2003)