Modulation of human gp91 ^<phox> gene expression by rickettsia infection

立克次体感染对人 gp91 ^<phox> 基因表达的调节

• 批准号: 14570240
• 负责人: NAKAMURA Michio
• 金额: $ 2.24万
• 依托单位: The Institute of Tropical Medicine, Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570240/
• 关键词: Ehrlihia; Rickettsia; gp91^<phox>; NADPH oxidase; p47^<phox>; Superoxide; Ehrlichia chaffeensis; 食細胞; 活性酸素; HGE; 分化

To escape from reactive oxygen species generated in response to infection, intracellular pathogens, such as Salmonellae and Anaplasma phagocytophilum, can modulate the expression and distribution of the phagocyte NADPH oxidase components.In the present study, we analyzed the effect of Ehrlichia chaffeensis infection on the ability of human monocytic THP-1 cells to produce superoxide anion. Phorbol ester-stimulated superoxide generation and mRNA for gp91^<phox> in paralel with mRNAs for p47^<phox>, and p67^<phox> were significantly increased in E. chaffeensis-infected THP-1 cells. These increases were also achieved in THP-1 cells cultured in medium conditioned by the E. chaffeensis-infected cells, indicating that bystander cells can be activated for superoxide generation and implicating soluble factors in the response. Heat-stable and -unstable factors represented one-third and two-thirds of this activity, respectively. These results suggest that immature human monocytic cells increase their ability to generate superoxide anion in response to E. chaffeensis infection regardless they have been directly invaded or not. Further studies have suggested that these factors are exclusively derived from the bacteria.

沙门氏菌和吞噬无浆菌等细胞内病原体可以调节吞噬细胞NADPH氧化酶组分的表达和分布，以逃避感染所产生的活性氧物种。佛波酯刺激THP-1细胞gp91；lt；Phox&gt；与p47；lt；Phox&gt；和p67^&lt；Phox&gt；mRNAs平行表达的超氧化物歧化产物和mRNA显著增加。在被查菲肠杆菌感染的细胞条件培养液中培养的THP-1细胞也出现了这些增加，表明旁观者细胞可以被激活以产生超氧化物，并在反应中涉及到可溶性因素。热稳定和不稳定因素分别占这一活动的三分之一和三分之二。这些结果表明，未成熟的人单核细胞增加了它们产生超氧阴离子的能力，以响应查菲埃希氏菌的感染，无论它们是否直接入侵。进一步的研究表明，这些因素完全来自细菌。

项目成果

M.Takata-Yahiro, Y.Fujii, et al.: "A novel GT-mismatch binding protein that recognizes strict DNA sequences with high affinity"Tohoku J.Exp.Med.. 200. 211-229 (2003)

M.Takata-Yahiro、Y.Fujii 等人：“一种以高亲和力识别严格 DNA 序列的新型 GT 错配结合蛋白”Tohoku J.Exp.Med.. 200. 211-229 (2003)

中村三千男: "好中球機能不全"臨床検査. 47・13. 1693-1701 (2003)

中村道雄：“中性粒细胞功能障碍”临床检查47・13（2003）。

Human monocytic cells upregulate superoxide-generating activity and mRNAs for its components in response to heat-stable and heat-unstable factors released to medium conditioned with Ehrlichia chaffeensis-infected THP-1 cells

人单核细胞上调超氧化物生成活性及其成分的 mRNA，以响应释放到查菲埃里希体感染的 THP-1 细胞条件培养基中的热稳定和热不稳定因子

• 发表时间: 2004
• 期刊: Acta Medica Nagasakiensia 49
• 作者: D.Gunasekara;Y.Yoshito;K.Yoshiie;N.Ohashi;M.Nakaniuraa
• 通讯作者: M.Nakaniuraa

Y.Fujii, A.Kumatori, M.Nakamura: "SATB1 makes a complex with p30c and represses gp91^<phox> promoter activity"Microbiol.Immunol.. 47・10. 803-811 (2003)

Y.Fujii、A.Kumatori、M.Nakamura：“SATB1 与 p30c 形成复合物并抑制 gp91^<phox> 启动子活性”Microbiol.Immunol.. 47・10 (2003)。