Lineage-specific expression of CYBB in leukocytes

白细胞中 CYBB 谱系特异性表达

基本信息

项目摘要

(1) Roles of PU.1 and HAF-1 for the expression of CYBBSingle base substitutions were systematically induced to the CYBB promoter and examined their effects on reporter activities and binding affinities to PU.1 and HAF-1. Data suggested that PU.1 is principal and HAF-1 supplementary. These also implied new CGDs and supplied ideally modified promoter sequences for maximal expression of the gene in the CGD phagocytes.(2) A new cw-elemement for the activation of CYBBWe discovered a new GATA-binding site on the CYBB promoter. This site is essential for the expression of the gene promoted by PU.1 and GATA-1.(3) A role of IRF-4 in B lymphocytesIRF-4 was suggested to inhibit CYBB that may account for the low expression of gp91phox in B lymphocytes.(4) Trials for GATA-1 gene transfection to phagocytes of the X-CGD patient with an abnormal CYBB promoterFor making gp91phox-deficient neutrophils/monocytes to gp91phox-normal eosinophils, we tried to transfect GATA-1 gene to the peripheral blood monocytes of a patient with X-linked bp-53T CGD patient, Efficiencies of liposomal and adenoviral transfections were inefficient but activated the gene nonspecifically suggesting a potential of the gene to be activated endogenously.(5) Identification of the epitope for 7D5, a monoclonal antibody raised against flavocytochrome b5587D5 epitope was molecular-biologically located to the extracellular peptide conformation of gp91phox. The antibody is now undoubtedly available for diagnosis of X-CGD and monitoring the efficiency of the gene ttransfer to CGD patients.
(1)PU.1和HAF-1对CYBB表达的作用系统地诱导CYBB启动子的单碱基取代,并检查它们对报告活性和对PU.1和HAF-1的结合亲和力的影响。数据表明,PU.1是主要的,HAF-1是补充。这些也意味着新的CGDs和提供理想的修饰的启动子序列的CGD吞噬细胞中的基因的最大表达。(2)激活CYBB的新顺式元件我们在CYBB启动子上发现了一个新的GATA结合位点。该位点对于由PU. 1和加塔-1促进的基因的表达是必需的。(3)IRF-4在B淋巴细胞中具有抑制CYBB的作用,这可能是B淋巴细胞中gp 91 phox表达降低的原因。(4)将加塔-1基因转染到具有异常CYBB启动子的X-CGD患者的吞噬细胞中的试验为了使gp 91 phox缺陷的中性粒细胞/单核细胞变为gp 91 phox正常的嗜酸性粒细胞,我们尝试将加塔-1基因转染到具有X连锁bp-53 T CGD患者的外周血单核细胞中,脂质体和腺病毒转染的效果是无效的,但非特异性地激活了该基因,这表明该基因有被激活的潜力内生的(5)抗黄细胞色素b5587 D5表位单克隆抗体7 D5的表位鉴定通过分子生物学方法将其定位于gp 91 phox的胞外肽构象。该抗体目前无疑可用于诊断X-CGD和监测CGD患者的基因转移效率。

项目成果

期刊论文数量(13)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Yamauchi, Akira: "Location of the epitope for 7D5, a monoclonal antibody raised against human favocytochrome b558, to the ertracellular peptide portion of primate gpqlphox"Microbiol Immunol. 45. 249-257 (2001)
Yamauchi,Akira:“7D5 表位的位置,7D5 是一种针对人黄细胞色素 b558 产生的单克隆抗体,与灵长类 gpqlphox 的细胞内肽部分”微生物免疫学。
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Kumatori, Atsushi: "Cooperation of STAT-1 and IRF-1 in interferon-r-induced transcription of the gpqlphox gene"J Biol Chem. 277. 9103-9111 (2002)
Kumatori, Atsushi:“STAT-1 和 IRF-1 在干扰素 r 诱导的 gpqlphox 基因转录中的合作”J Biol Chem。
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Kumatori A, Yang D, Suzuki S, Nakamura A.: "Cooperation of SYAT-1and IRF-1 in interferon-α-induced transcription of the gp91^<Phox>gene."J Biol Chem. 277・11. 9103-9111 (2002)
Kumatori A、Yang D、Suzuki S、Nakamura A.:“SYAT-1 和 IRF-1 在干扰素 α 诱导的 gp91^<Phox> 基因转录中的合作。”J Biol Chem 277・11。 (2002)
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Akira Yamauchi: "Location of the epitope for 705, a monoclonal antibody raised against human flavocytochrome b558, to the extracellular peptide portion of primate gp91^<phox>"Microbiol.Immunol. 45・3(未定). (2001)
Akira Yamauchi:“针对人黄细胞色素 b558 的单克隆抗体 705 的表位定位于灵长类动物 gp91^<phox> 的胞外肽部分”Microbiol.Immunol. (2001)。
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Kumatori Atsushi: "Cooperation of STAT-1 and IRF-1 in interferon-gamma-induced transcription of the gp91(phox) gene."J Biol Chem. 277. 9103-11 (2002)
Kumatori Atsushi:“STAT-1 和 IRF-1 在干扰素 γ 诱导的 gp91(phox) 基因转录中的合作。”J Biol Chem。
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NAKAMURA Michio其他文献

Successful Treatment of Ruptured Dissecting Vertebral Artery Aneurysms through Proximal Occlusion of the Vertebral Artery along with Reconstruction of the Posterior Inferior Cerebellar Artery: Two Case Reports
通过椎动脉近端闭塞结合小脑后下动脉重建成功治疗破裂的椎动脉夹层动脉瘤:两个病例报告
  • DOI:
    10.2335/scs.49.401
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    0
  • 作者:
    NAKAMURA Michio;MIYAZAKI Tadashi;FUSE Yoshihiko;ADACHI Akihiko;YONEYAMA-SARNECKY Tomoko;OZAKI Koh
  • 通讯作者:
    OZAKI Koh

NAKAMURA Michio的其他文献

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{{ truncateString('NAKAMURA Michio', 18)}}的其他基金

Influence of Klotho produced from transplanted kidneys on mineral metabolism and graft/patient survival in kidney transplant patients
移植肾产生的 Klotho 对肾移植患者矿物质代谢和移植物/患者生存的影响
  • 批准号:
    25461962
  • 财政年份:
    2013
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Comparison of culture between two adjacent wild chimpanzee groups
相邻两个野生黑猩猩群体的培养比较
  • 批准号:
    21770262
  • 财政年份:
    2009
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Cloning and characterization of novel GT-mismatch DNA binding protein
新型 GT 错配 DNA 结合蛋白的克隆和表征
  • 批准号:
    16590248
  • 财政年份:
    2004
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Modulation of human gp91 ^<phox> gene expression by rickettsia infection
立克次体感染对人 gp91 ^<phox> 基因表达的调节
  • 批准号:
    14570240
  • 财政年份:
    2002
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Regulation by PU.1 of CYBB Expression
PU.1对CYBB表达的调节
  • 批准号:
    09671123
  • 财政年份:
    1997
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Generation and Role of Active oxygens in Cells
细胞中活性氧的产生和作用
  • 批准号:
    05044180
  • 财政年份:
    1993
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for international Scientific Research

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