Morphological changes of endothelial cells and pharmaceutical actions of drugs and poisons using the cells.

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Toxicity of drugs and poisons shows as lethal dose in human. In this study, relationship between the lethal dose and dose of the cell death was examined using human endothelial cells(HUVEC). Death of HUVEC judged with morphological changes in culture fluids mixed with drugs and poisons. Doses of the cell death using methamphetamine(MA), cocaine, phenobarbital(PB), paraquat(PQ) showed 5,5,5 and 0.1 mM, respectively. The other 57 drugs and poisons were screened with the same method. The most poisonous substance was arsenite sodium (0.01mM) and the least poisonous one was methanol(2M). By the references, lethal doses of human(including presumption values) in MA, cocaine, PB and PQ were 1.3-20,7-10,14-200 and 14-40mg/kg, respectively. To compare the values between lethal dose and dose of cell death, the unit made mg/L of mg/kg. The concentrations of MA, cocaine, PB and PQ were 0.007-0.1,0.002-0.003,0.06-0.9 and 0.05-0.16mM, respectively. In MA, cocaine and PB lethal doses of human showed 1/6 to 1/700 comparing to cell death of HUVEC. In PQ, however, the dose of human was same level as cell death of HUVEC. In the same manner, 49 substances were plotted between lethal dose of human and the cell death of HUVEC. When the relation line was made, the correlation coefficient(r) was 0.310, indicating poor relationship. In conclusion, morphological changes of HUVEC showed poor relationship to lethal dose of human in total 61 drugs and poisons examined. Further examination needs to determine the relationship between lethal dose and endothelial cell death in human.