The study of systematic method in identifying various populatins from the forensic specimens using polymorphic DNA markers.

利用多态性DNA标记从法医标本中鉴定各种种群的系统方法研究。

• 批准号: 14570384
• 负责人: OTA Masao
• 金额: $ 2.24万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570384/
• 关键词: HLA gene polymorphism; HLA haplotype; PCR-SPP method; Forensic specimens; Japanese population; Distribution of HLA gene frequency; Personal identification; Microsatellite

The systematic procedure for identifying the forensic specimens, whether they are originated from Japanese population or other ethnic populations, is helpful for resolving a criminal investigation happened in Japan. First, we studied the usefulness of HLA DNA polymorphisms to differentiate Japanese population from the numerous populations in the word. We listed characteristic and rare or absent HLA genes and haplotypes in Japanese population from the 11^<th> International HLA data, and made available tables to compare Japanese and other ethnic groups. Second, we validated the HLA-SPP(sequence specific primer and probe) typing method for forensic specimens, especially from extremely small amount of DNA extracted such as a single hair, a piece of nail, aged blood stains, fromalin-fixed and paraffin-embedded tissues. This PCR-SPP method has made it possible to type HLA alleles even a very small amount of DNA. Third, we demonstrated a power of discrimination on microsatellite markers located in the HLA region from the results that allele types of microsatellite were different at several loci between the same HTCs (homozygous typing cell), and also different among persons with the same frequent haplotype (A : 2402-C^* 1202-B^*5201-DRB1^*1502-DQB1^*0601) in Japanese population. Theses results supported HLA DNA polymorphisms as a useful tool to identify the forensic specimens originated from Japanese or not.

系统的法医标本鉴定程序，无论是来自日本人还是其他民族，都有助于解决发生在日本的刑事调查。首先，我们研究了人类白细胞抗原DNA多态在区分日本人群和世界上众多人群中的作用。我们从第11次国际人类白细胞抗原数据中列出了日本人特有的、稀有或缺失的基因和单倍型，并制作了表格来比较日本人和其他民族。其次，我们验证了序列特异性引物和探针(Sequence Special Primer and Probe，HSA-SPP)分型方法的有效性，特别是从极少量的DNA提取中，如一根头发、一块指甲、陈年血迹、石蜡包埋组织和固定组织。这种PCR-SPP方法使得即使是非常少量的DNA也能对人类白细胞抗原等位基因进行分型。在日本人群中，同一纯合子分型细胞(HTC)的微卫星在多个基因座上的等位基因类型不同，相同频率单倍型(A：2402-C^*1202-B^*5201-DRB1^*1502-DQB1^*0601)的人群也不同。这些结果支持将HLADNA多态性作为鉴定法医标本来源是否来自日本的有用工具。

项目成果

Takayanagi K, Asamura H, Tsukada K, Ota M, Fukushima H: "Hair analysis for human and species identification."DNA polymorphism (Japanese). 11. 143-146 (2003)

Takayanagi K、Asamura H、Tsukada K、Ota M、Fukushima H：“用于人类和物种鉴定的头发分析。”DNA 多态性（日语）。

Akamatu T, Sano K, Tsugane K, Ota M: "Identification of mixed-up tissue specimens : immunohistochemical staining of blood group substances and application of short tandem repeat of genomic DNA"Shokaki Naishikyo (Japanese). 14. 385-389 (2002)

Akamatu T、Sano K、Tsugane K、Ota M：“混合组织标本的鉴定：血型物质的免疫组织化学染色和基因组 DNA 短串联重复的应用”Shokaki Naishikyo（日语）。

Okamoto K, 他9名: "Identification of I kappa BL as the second major histocompatibility complex-linked susceptibility locus for rheumatoid arthritis"Am J Hum Genet. 72. 303-312 (2003)

Okamoto K 和其他 9 人：“鉴定 I kappa BL 作为类风湿性关节炎的第二个主要组织相容性复合物相关易感基因座”Am J Hum Genet。72. 303-312 (2003)

Mizuki N, 他12名: "Analysis of microsatellite polymorphism around the HLA-B locus in Iranian patients with Behcet's disease"Tissue Antigens. 60. 396-399 (2003)

Mizuki N 等 12 人：“伊朗白塞病患者 HLA-B 基因座周围的微卫星多态性分析”组织抗原 60. 396-399 (2003)。

Mizuki N, Ota M, 他11名: "Analysis of microsatellite polymorphism around the HLA-B loacs m Iranian patients with Be hcet's disease"Tissue Antigens. 60. 396-399 (2002)

Mizuki N、Ota M 和其他 11 人：“伊朗 Be hcet 病患者 HLA-B 基因周围的微卫星多态性分析”组织抗原 60. 396-399 (2002)。