The study of HLA-DNA typing technique applicable to forensic investigation

我调查的适用性HLA-DNA分型使用PCR技术为基础的法医调查。首先，将PCR-SSP和PCR-RFLP相结合的HLA-class II分型技术应用于法医学实践。PCR-SSP分型与HLA-DR血清学分型同样有效。PCR-RFLP分型用于更精确地定义编码DR 2、DDR4和DR 8的DRB 1等位基因。此外，我还采用了巢式或半巢式PCR技术来提高该系统的灵敏度。其次，研究了PCR-SSP方法在HLA Ⅰ类分型中的适用性。本研究制备了96种引物混合物，用于扩增与血清学Ⅰ类抗原相对应的HLA-A、-B和-C等位基因，从法医学检材中提取的DNA样本中可以用这些方法进行HLA-DNA分型，但DNA的质量和数量是成功的关键。

I investigated the applicability of HLA -DNA typing using PCR-based technique to forensic investigations. First, I applied the HLA-class II typing combined With PCR-SSP and PCR-RFLP to forensic practice. PCR-SSP typing was an effective as serological HLA-DR typing in determining DR types. PCR-RFLP typing was used for more precise definition of DRB1 alleles encoding DR2, DDR4, and DR8. Furthermore I used the nested or semi-nested PCR technique to increase the sensitivity of this system. Second, I studied the applicability of HLA-class I typing using PCR-SSP method. I made 96 kinds of primer mixture to amplify HLA-A,-B and-C alleles corresponding to serological class I antigens.HLA-DNA typing by these methods were possible from DNA samples extracted from forensic samples, but both quality and quantity of DNA were important for successful.