The study of HLA-DNA typing technique applicable to forensic investigation

我调查了HLA -DNA分型的适用性使用pcr为基础的技术法医调查。首先，我将hlaⅱ类分型结合PCR-SSP和PCR-RFLP应用于法医实践。PCR-SSP分型与血清学HLA-DR分型在诊断DR类型方面效果相同。PCR-RFLP分型用于更精确地定义编码DR2、DDR4和DR8的DRB1等位基因。此外，我使用嵌套或半嵌套PCR技术来提高该系统的灵敏度。其次，研究了PCR-SSP法hla I类分型的适用性。制作96种引物混合物，扩增血清学ⅰ类抗原对应的HLA-A、-B和c等位基因。通过这些方法从法医样本中提取的DNA样本中进行HLA-DNA分型是可能的，但DNA的质量和数量对于成功至关重要。

I investigated the applicability of HLA -DNA typing using PCR-based technique to forensic investigations. First, I applied the HLA-class II typing combined With PCR-SSP and PCR-RFLP to forensic practice. PCR-SSP typing was an effective as serological HLA-DR typing in determining DR types. PCR-RFLP typing was used for more precise definition of DRB1 alleles encoding DR2, DDR4, and DR8. Furthermore I used the nested or semi-nested PCR technique to increase the sensitivity of this system. Second, I studied the applicability of HLA-class I typing using PCR-SSP method. I made 96 kinds of primer mixture to amplify HLA-A,-B and-C alleles corresponding to serological class I antigens.HLA-DNA typing by these methods were possible from DNA samples extracted from forensic samples, but both quality and quantity of DNA were important for successful.