The study of HLA-DNA typing technique applicable to forensic investigation

HLA-DNA分型技术应用于法医侦查的研究

• 批准号: 09670434
• 负责人: OTA Masao
• 金额: $ 1.79万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670434/
• 关键词: HLA; Class I genes; Class II genes; Forensic samples; DNA typing; PCR-SSP; PCR-RFLP; nested PCR; DNA タイピング; PCR-SSO; PCR-SSCP; HLAクラスI遺伝子; HLA-クラスII遺伝子

I investigated the applicability of HLA -DNA typing using PCR-based technique to forensic investigations. First, I applied the HLA-class II typing combined With PCR-SSP and PCR-RFLP to forensic practice. PCR-SSP typing was an effective as serological HLA-DR typing in determining DR types. PCR-RFLP typing was used for more precise definition of DRB1 alleles encoding DR2, DDR4, and DR8. Furthermore I used the nested or semi-nested PCR technique to increase the sensitivity of this system. Second, I studied the applicability of HLA-class I typing using PCR-SSP method. I made 96 kinds of primer mixture to amplify HLA-A,-B and-C alleles corresponding to serological class I antigens.HLA-DNA typing by these methods were possible from DNA samples extracted from forensic samples, but both quality and quantity of DNA were important for successful.

我调查的适用性HLA-DNA分型使用PCR技术为基础的法医调查。首先，将PCR-SSP和PCR-RFLP相结合的HLA-class II分型技术应用于法医学实践。PCR-SSP分型与HLA-DR血清学分型同样有效。PCR-RFLP分型用于更精确地定义编码DR 2、DDR4和DR 8的DRB 1等位基因。此外，我还采用了巢式或半巢式PCR技术来提高该系统的灵敏度。其次，研究了PCR-SSP方法在HLA Ⅰ类分型中的适用性。本研究制备了96种引物混合物，用于扩增与血清学Ⅰ类抗原相对应的HLA-A、-B和-C等位基因，从法医学检材中提取的DNA样本中可以用这些方法进行HLA-DNA分型，但DNA的质量和数量是成功的关键。

项目成果

Ota M.,et al.: "Validation of HLA-PR locustyping in forensic specimens by combining PCR-SSP with PCR-RFLP" J Forensic Sei. 42. 929-934 (1997)

Ota M. 等人：“通过结合 PCR-SSP 与 PCR-RFLP 验证法医标本中的 HLA-PR 基因座分型”J Forensic Sei。

Inoko,H et al.: "Genetic diversity of HLA functional and medical imphication" EDK Medical and Scientific International Publishen, 10 (1977)

Inoko,H 等人：“HLA 功能和医学暗示的遗传多样性”EDK Medical and Scientific International Publishen，10 (1977)

太田正穂 他: "HLAタイプ法-DNA解析" 臨床免疫. 29. 155-166 (1997)

Masaho Ota 等：“HLA 分型方法-DNA 分析”《临床免疫学》29. 155-166 (1997)。

Ota M., Ando H., Inoko H.: "HLA typing method : DNA typing. (Japanese)" RINSHOU MENEKI. 29. 155-166 (1997)

Ota M.、Ando H.、Inoko H.：“HLA 分型方法：DNA 分型。（日语）” RINSHOU MENEKI。

太田正穂: "新臨床免疫実験操作法" 科学評論社, 12 (1977)

Masaho Ota：“新临床免疫学实验程序” Kagaku Hyoronsha，12（1977）