A study of the pigment which inhibits enzymatic reaction

据了解，在头发、人骨等法医或人类学样本中，如果有色素渗入，由于聚合酶链反应无法进行，因此很难进行DNA分型。我进行这项研究的目的是建立从含有色素的样品中进行DNA分型的方法。起初，我试图去除染发剂中抑制PCR反应的色素，但不幸的是没有找到有效的方法。然而，即使是用永久性染发剂处理过的头发，很明显，PCR是成功的，因为它使用的是该方法提取的模板dna，而没有溶解毛柱皮层。另一方面，我关注了埋藏土壤中抑制PCR的天然色素。然后，我尝试在不受这些色素成分酶抑制的情况下，从古代骨骼中提取DNA。因此，即使从染色的骨头中提取DNA，我独创的用硅粉提取DNA的方法也可以提高DNA分型的成功率。在这项研究中，我成功地克服了大多数天然色素和合成色素的酶抑制作用。

When some pigment infiltrate into a forensic or an anthropological sample such as a hair and a human bone, it is known that DNA typing is impossible in many cases because PCR reaction does not advance. I carried out this study for the purpose of establishing methods for DNA typing from the sample in which the pigment was included.At first, I tried to remove the pigment of hair dye which inhibits PCR reaction, but could not unfortunately find effective methods to do it. However, even in the case of a hair treated with perpetual hair dye, it became clear that PCR is successful by using template DNAs extracted by the method without the dissolution of the pilar cortex. On the other hand, I paid my attention to natural pigment, which inhibited PCR, in the burial soil. Then, I tried to extract DNA from ancient bone without enzyme inhibition by these pigment ingredients. As a result, even in DNA extracted from the dyed bones, a success rate of DNA typing could be raised by my original method of DNA extraction using silica powder.In this study, I succeeded in overcoming most, of the enzyme inhibition with natural and synthetic pigments.