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The analysis of carcinogenesis mechanism by the infection of HCV and HBV

HCV和HBV感染致癌机制分析

基本信息

批准号：
14570435
负责人：
NURIYA Hideko
金额：
$ 2.24万
依托单位：
TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570435/
关键词：
WRN helicase HCV HBV Patients' liver tissue HBV, HCV重複感染 DNA helicase 発癌

项目摘要

The higher risk of developing hepatocellular carcinoma (HCC) rises with HBV and HCV co-infection. To make clarify this phenomenon, we noticed of the alteration of DNA helicase (especially, WRN helicase). The amount of expression of WRN helicase protein (WRN) in the liver tissue was examined by western blotting method. There was more amount of WRN protein of the cancerous region than a non-cancerous region in HCC of HCV (-) and HCV (+). And there was more amount of WRN protein of HCC of HCV (+) than HCV (-). Moreover we analyzed by immunostaining method, WRN protein was detected in the nucleus and the surroundings of the nucleus and not detected there in the non-cancerous region of HCC of HCV (-). WRN protein was detected in the nucleus and surroundings of the nucleus in the non-cancerous region of the HCC of HCV (+). But it was not detected in the nucleus of the cancerous region of HCV (+). These results showed that the amounts of expression of WRN protein increased in HCC of HCV (+) more than HCV (-). Although it is the possibility to WRN protein is not able be transported to the nucleus in the cancerous region. In HBV and HCV co-infected HCC, WRN protein was detected much more weakly than HCV single infection. It is the possibility that WRN protein be transported to the nucleus is suppressed strongly much more. These results indicated it the possibility that HCV changed to localization of WRN protein in heoatocytes.

发生肝细胞癌（HCC）的风险随着HBV和HCV合并感染而升高。为了阐明这一现象，我们注意到DNA解旋酶（尤其是WRN解旋酶）的改变。用免疫印迹法检测肝组织WRN解旋酶蛋白（WRN）的表达量。在HCV阴性和HCV阳性的HCC中，癌区WRN蛋白的表达量明显高于非癌区。HCV（+）组肝癌组织中WRN蛋白表达量明显高于HCV（-）组。免疫组化分析表明，WRN蛋白在HCV（-）肝癌细胞核及核周围表达，而在非癌区无表达。在HCV（+）肝癌组织的非癌区，WRN蛋白在细胞核及核周围表达。而在HCV（+）癌区细胞核中未检测到。结果表明，HCV（+）肝癌组织中WRN蛋白的表达量较HCV（-）肝癌组织中WRN蛋白的表达量增加。虽然WRN蛋白有可能不能被转运到癌组织的细胞核中。在HBV和HCV合并感染的HCC中，WRN蛋白的检测比HCV单独感染弱得多。WRN蛋白被转运到细胞核的可能性被大大抑制。这些结果提示HCV可能改变为WRN蛋白在肝细胞中的定位。