Mechanism of T/NKT cells activation in granuloma of Crohn's disease

克罗恩病肉芽肿中T/NKT细胞活化机制

• 批准号: 14570496
• 负责人: MASTUMOTO Takayuki
• 金额: $ 1.86万
• 依托单位: Hyogo College of Medicine (2004)Osaka City University (2002-2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570496/
• 关键词: Crohn's disease; Chemokines; T cells; Dendritic cells; Homing receptors; MAdCAM-1; 肉芽腫; 疾患特異抗原; T細胞抗原レセプター; インテグリン; NKT細胞; ボーミングレセプター; 粘膜免疫

Crohn's disease is thought to be related to the impaired immune reactions of macrophage lineage cells, which typically causes Th1-type reactions and granuloma formations in involved tissue. Although NOD2 gene polymorphisms are reported in patients with Crohn's disease in Western country, there is no SNP manifestation in Japanese patients. The aim of this study is to conduct the mechanism of such Th1 activation thorough impaired homing of T cells and possible role of NKT cells in Crohn's disease.1.In Crohn's disease, number of vascular endothelium expressing MAdCAM-1, one of the important molecules in mucosal immune systems, are increased compared to that of controls and ulcerative colitis.2.Memory T cells expressing Th1 cytokines and Ki-67, one of the proliferation related antigens are increased around such vessels.3.ELC and SLC, which are important chemokines in T cell migration are often observed around T cell infiltration in Crohn's disease.These results suggests the important roles of mucosa associated T cell activation and infiltration in the chronic active inflammation and granulomatous Th1 type reaction in Crohn's disease, possibly induced by the disease specific antigens.

克罗恩病被认为与巨噬细胞谱系细胞的免疫反应受损有关，这通常会导致Th 1型反应和相关组织中的肉芽肿形成。尽管在西方国家的克罗恩病患者中报道了NOD 2基因多态性，但在日本患者中没有SNP表现。本研究的目的是通过T细胞归巢受损和NKT细胞在克罗恩病中的可能作用来进行这种Th 1激活的机制。1.在克罗恩病中，表达粘膜免疫系统中重要分子之一MAdCAM-1的血管内皮细胞的数量与对照组和溃疡性结肠炎相比增加。2.表达Th 1细胞因子和Ki-67的记忆T细胞，3.克罗恩病患者T细胞浸润周围常出现ELC和SLC，它们是T细胞迁移的重要趋化因子，提示粘膜相关的T细胞活化和浸润在克罗恩病的慢性活动性炎症和肉芽肿性Th 1型反应中起重要作用，可能由疾病特异性抗原诱导。

项目成果

Adsorptive monocyte-granulocyteapheresis(M-GCAP) for refractory Crohn's disease.

吸附性单核细胞-粒细胞分离术（M-GCAP）治疗难治性克罗恩病。

• 发表时间: 2004
• 期刊: J Clin Apheresis 19
• 作者: Kusaka T;Matsumoto T;et al.
• 通讯作者: et al.

Immunopathology of inflammatory bowel disease and roles of the mucosal immune system

炎症性肠病的免疫病理学和粘膜免疫系统的作用

• 发表时间: 2002
• 期刊: Pathology Case Reviews 7
• 作者: Nagura H;Matsumoto T
• 通讯作者: Matsumoto T

Oshitani N, Kamata N, Ooiso R, Kawashima D, Inagawa M, Sogawa M., Iimuro M, Jinno Y, Watanabe K, Higuchi K, Matsumoto T, Arakawa T: "Outpatient treatment of moderataly severe active ulcerative colitis with pulsed steroid therapy and conventional steroid t

Oshitani N、Kamata N、Ooiso R、Kawashima D、Inakawa M、Sokawa M.、Iimuro M、Jinno Y、Watanabe K、Higuchi K、Matsumoto T、Arakawa T：“用脉冲类固醇疗法门诊治疗中重度活动性溃疡性结肠炎

Adsorptive monocyte-granulocyteapheresis (M-GCAP) for refractory Crohn's disease.

吸附性单核细胞-粒细胞分离术 (M-GCAP) 用于治疗难治性克罗恩病。

• 发表时间: 2004
• 期刊: J Clin Apheresis 19
• 作者: Kusaka T;Matsumoto T et al.
• 通讯作者: Matsumoto T et al.

Comprehensive analysis of intestinal cytokine messenger RNA profile by real-time quantitative polymerase chain reaction in patients with inflammatory bowel disease.

• DOI: 10.3892/ijmm.11.2.175
• 发表时间: 2003-02
• 期刊: International journal of molecular medicine
• 影响因子: 5.4
• 作者: Yoshinori Sawa;N. Oshitani;K. Adachi;K. Higuchi;T. Matsumoto;T. Arakawa