Distribution and characterization of IgG Fc binding protein in various mucin producing cells

IgG Fc 结合蛋白在各种粘蛋白产生细胞中的分布和表征

• 批准号: 14570506
• 负责人: OGATA Haruhiko
• 金额: $ 2.18万
• 依托单位: KEIO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570506/
• 关键词: mucosal defense; mucin; IgG Fc binding protein; intestinal mucosal immunology

Immunohistochemical localisation of Fc binding protein (FcBP), using monoclonal antibodies against FcBP was conducted in various mucin-producing tissues. The binding activity of FcBP in mucus extracted from mucin-producing tissues was also examined by immunodotblot and immunoprecipitation using these monoclonal antibodies. Inhibition of complement mediated haemolysis by FcBP was investigated using sheep red blood cells (SRBC) and anti-SRBC IgG. The immunohistochemical study revealed that mucin-secreting cells in the colon, small intestine, gall bladder, cystic duct, choledochus, bronchus, submandibular gland, and cervix uteri contained FcBP, and immunodotblot and immunoprecipitation analysis using IgG and monoclonal antibodies demonstrated that the fluids secreted by these cells were capable of binding IgG. Mucin producing cells of the conjunctiva did not express FcBP molecules or bind to IgG. The surface mucus cells in the stomach were variably positive for FcBP. Perhaps because of proteolytic degradation, FcBP in gut lavage fluid did not have IgG binding activity, although this activity was present in the mucus covering the colon. FcBP suppressed complement mediated haemolysis of SRBC. Thus FcBP is widely expressed on mucosal surfaces and in external secretions. It is functionally intact in several fluids. These findings lend support to the concept that FcBP is an important component of mucosal immunological defenses.

免疫组织化学定位的Fc结合蛋白（FcBP），使用单克隆抗体对FcBP进行了各种粘蛋白生产组织。从产生粘蛋白的组织中提取的粘液中的FcBP的结合活性也使用这些单克隆抗体通过免疫斑点印迹和免疫沉淀来检查。使用绵羊红细胞（SRBC）和抗SRBC IgG研究了FcBP对补体介导的溶血的抑制作用。免疫组化研究表明，结肠，小肠，胆囊，胆囊管，胆总管，支气管，颌下腺，子宫颈中的粘蛋白分泌细胞含有FcBP，免疫斑点印迹和免疫沉淀分析使用IgG和单克隆抗体证明，这些细胞分泌的液体能够结合IgG。结膜的粘蛋白产生细胞不表达FcBP分子或与IgG结合。胃表面粘液细胞呈FcBP阳性。可能由于蛋白水解降解，肠道灌洗液中的FcBP不具有IgG结合活性，尽管这种活性存在于覆盖结肠的粘液中。FcBP抑制补体介导的SRBC溶血。因此，FcBP广泛表达于粘膜表面和外部分泌物中。它在几种液体中功能完整。这些发现支持了FcBP是粘膜免疫防御的重要组成部分的概念。

项目成果

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