Anti-neural antibodies induced by brain injury and analysis of their antigen

脑损伤诱导的抗神经抗体及其抗原分析

• 批准号: 14570587
• 负责人: INUZUKA Takashi
• 金额: $ 1.98万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570587/
• 关键词: anti-neural antibodies; brain injury; autoimmunity; aging; 免疫ブロット法; アルツハイマー型痴呆

Objects of our studies were detection and analysis of anti-neural antibodies induced by experimental brain injury, which may elucidate significance of the specific anti-neural antibodies in some neurological disease.1.Spraque-Dawley female rats (6 month, 2 year age) were used, Bilateral stub wounds were made with a dissecting knife, Sera from rats were collected before and 4 weeks after operation. Sera were examined if they contained newly synthesized anti-neural antibodies by the Western blot using rat cerebrum, brainstem and liver as antigens.2.Sera from 3 patients with cerebral infarction were collected at the day of onset and one month later, 3 pairs Non pair had that, None newly synthesized anti-neural antibody was detected in rat or human. Sensitivity of the system, limited amount of blood from rats are thought of the possibility to fail detection of antibody. Inflammatory insult to brain should be examined if it cause the anti-neural antibodies.

本研究的目的是检测和分析实验性脑损伤诱导的抗神经抗体，以阐明特异性抗神经抗体在某些神经系统疾病中的意义。1.选用6月龄、2岁的Spraque-Dawley雌性大鼠，用解剖刀制作双侧残端创面，分别于术前和术后4周采集血清。以大鼠大脑、脑干和肝脏为抗原，用Western印迹法检测血清中是否含有新合成的抗神经抗体。2.收集3例脑梗塞患者发病当天和1个月后的血清，其中3对未检测到新合成的抗神经抗体，大鼠和人均未检测到新的抗神经抗体。该系统的敏感性，有限的大鼠血液被认为是抗体检测失败的可能性。如果炎性侮辱引起抗神经抗体，则应检查其对大脑的损害。