Identification of muscle specific autoantibodies for myasthenic syndromes and myositis by proteomic analysis

通过蛋白质组学分析鉴定肌无力综合征和肌炎的肌肉特异性自身抗体

• 批准号: 20590993
• 负责人: INUZUKA Takashi
• 金额: $ 2.83万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590993/
• 关键词: プロテオミクス; 抗筋抗体; 炎症性筋炎; バイオマーカー; myosin-binding protein; pyruvate kinase isozyme; LC-MS; MSシステム; 二次元ブロット; 解糖系酵素; 質量分析

We identified two novel antibodies against muscle-specific components in sera of idiopathic inflammatory myopathies (IIMs) patients by proteomic analysis. These target antigens were myosin-binding protein C (MyBP C) and pyruvate kinase isozyme M1/M2 (PK M1/M2). MyBP C belongs to the immunoglobulin superfamily. PK catalyzes the conversion of phosphoenolpyruvate with regeneration of ATPs. Both molecules have been reported as possible myositogenic antigen for experimental autoimmune polymyositis.The number of patients with the anti- MyBP C antibody was significantly higher among the IIM patients (9 of 10) than among the healthy control (1 of 10) and myotonic dystrophy (MyD) patients (5 of 11). The number of patients with the anti-PK M1/M2 antibody was significantly higher among the IIM patients (5 of 10) than among the healthy control (0 of 10) and myotonic dystrophy (MyD) patients (2 of 11). The sensitivity of this combination assay for diagnosis IIM was 50% and specificity was 80%. The sensitivity of this combination assay for diagnosis polymyositis was 71% and specificity was 92%. Therefore we propose that this combination assay is a candidate for further exploration as a diagnostic marker of IIM, particularly polymyositis.To clarify the pathogenic roles of anti- MyBP C and PK M1/M2 antibodies in IIM, we must perform more experiments such as using animal models in which these antibodies are passively administrated.

我们通过蛋白质组学分析鉴定了两种针对特发性炎性肌病（IIMs）患者血清中肌肉特异性成分的新型抗体。这些靶抗原是肌球蛋白结合蛋白C（MyBP C）和丙酮酸激酶同工酶M1/M2（PK M1/M2）。MyBP C属于免疫球蛋白超家族。PK催化磷酸烯醇丙酮酸的转化，并再生ATP。IIM患者中具有抗MyBP C抗体的患者数量（9/10）显著高于健康对照（1/10）和强直性肌营养不良（MyD）患者（5/11）。IIM患者（5/10）中具有抗PK M1/M2抗体的患者数量显著高于健康对照（0/10）和强直性肌营养不良（MyD）患者（2/11）。该联合检测诊断IIM的敏感性为50%，特异性为80%。联合检测诊断多发性肌炎的敏感性为71%，特异性为92%。因此，我们建议，这种联合检测是一个候选人，为进一步探索作为诊断标志物的IIM，特别是polymyositis.To澄清的致病作用，抗MyBP C和PK M1/M2抗体在IIM，我们必须进行更多的实验，如使用这些抗体的动物模型中被动管理。

项目成果

Anti-Endothelial Cell Antibodies in the Elderly with Extended White Matter Hyperintensity.

患有长期白质高信号的老年人的抗内皮细胞抗体。

• 发表时间: 2011
• 作者: A. Kimura;T. Sakurai;M. Yamada;A. Koumura;Y. Hayashi;Y. Tanaka;I. Hozumi;H. Ohtaki;M. Chousa;M. Takemura;M. Seishima;and T. Inuzuka.
• 通讯作者: and T. Inuzuka.

High prevalence of autoantibodies against phosphoglycerate mutase l in patients with autoimmune central nervous system diseases

自身免疫性中枢神经系统疾病患者中磷酸甘油酸变位酶 l 自身抗体的高患病率

• 发表时间: 2010
• 期刊: Journal of Neuroimmunology 219
• 作者: Kimura A;Sakurai T;Yamada M;Hayashi Y;Tanaka Y;Hozumi I;Tanaka R;Takemura M;Seishima M;Inuzuka T
• 通讯作者: Inuzuka T

Identification of Antibodies Against Brain Antigen in Sera of Patients with Neuropsychiatric Systemic lupus erythematosus.

神经精神系统性红斑狼疮患者血清中抗脑抗原抗体的鉴定。

• 发表时间: 2009
• 作者: 木村暁夫;櫻井岳郎;山田恵;林祐一;田中優司;保住功;犬塚貴
• 通讯作者: 犬塚貴

Motor-dominant CIDP患者の臨床的検討

运动主导型 CIDP 患者的临床回顾

• 发表时间: 2010
• 作者: 木村暁夫;櫻井岳郎;山田恵;香村彰宏;林祐一;田中優司;保住功;犬塚貴
• 通讯作者: 犬塚貴

High levels of copper, zinc, iron and magnesium, but not calcium, in the cerebrcspinal fluid of patients with Fahr's disease.

法赫尔病患者的脑脊液中铜、锌、铁和镁含量较高，但钙含量不高。

• 发表时间: 2010
• 期刊: Case Report of Neurology
• 作者: Hozumi I;Koumura A;Kimura A;Hasegawa T;Honda A;Hayashi Y;Hashimoto K;Yamada M;Sakurai T;Tanaka Y;Satoh M;Inuzuka T
• 通讯作者: Inuzuka T