Establishment of the detection system for anti-neural antibodies in the patients with nervous disease and studies on the antigens recognized by anti-neural antibodies

我们建立了抗神经抗体的检测系统，采用免疫印迹、免疫组织化学和ELISA方法。1997-98年间检测了300多份样本。除已知的抗Hu、抗Yo、抗MAG等自身抗体外，在某些特定的神经系统疾病中未发现免疫染色的特征条带或模式。我们对21例抗Hu抗体阳性的副肿瘤神经综合征患者进行了抗核抗体的临床分析和表征。对于抗HU阳性的PNS，临床表现各不相同，如感觉神经病。小细胞肺癌常伴有抗HU阳性的PNS.抗HU抗体的检测提示某些特殊类型的隐匿性恶性肿瘤，有助于寻找恶性肿瘤。这些患者除了抗HU抗体外，通常还有核抗体。由于抗HU抗体识别的抗原含有RNA识别基序，我们利用PC12细胞培养系统检测了抗HU抗体的作用。在培养上清液中加入抗Hu抗体或用微量注射器注入细胞内，均未观察到明显的细胞损伤。我们发现，HUD反义寡核苷酸对HUD蛋白提呈和PC12向神经细胞分化有抑制作用，而抗HU抗体对NGF的添加可能与分化有关。

We established the detection system for anti-neural antibodies, using immunoblot, immunohistochemical approach and ELISA.Over 300 samples were examined in 1997-98. Thirty percent of those contained auto-antibodies, one-third of it reacted with neural antigens.We could not find any characteristic band or pattern of immunostaining in some specific neurological disease except some known auto-antibodies such as anti-Hu, anti-Yo and anti MAG antibodies.We studied clinical analysis and characterization of anti-nuclear antibodies in 21 patients of paraneoplastic neurological syndrome with anti-Hu antibodies. Clinical features were variable regarding anti-Hu positive PNS, such as sensory neuropathy. Small cell lung cancer was often associated with anti-Hu positive PNS.Detection of anti-Hu antibodies suggest some specific kinds of occult malignancy and are helpful when searching for malignancy. These patients often have nuclear antibodies in addition to anti-Hu antibodies. As recognized antigens by anti-Hu antibodies contain RNA recognition motif, we examined effects of anti-Hu antibodies using culture system of PC12 cell. Anti-Hu antibodies were added in culture medium or injected into the cell by micro injector, Significant cell injury were not observed. We found the inhibition effect of HuD anti-oligonucleotide but not anti-Hu antibodies on presentation of HuD protein and differentiation of PC12 to neuronal cell adding of NGF.HuD protein may relate to differentiation.