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Expression of E-selectin ligand and fucosyltransferase VII occurring with differentiation into Th1 and Th2

E-选择素配体和岩藻糖基转移酶 VII 的表达伴随分化为 Th1 和 Th2

基本信息

批准号：
14570821
负责人：
HAYAKAWA Kazuhito
金额：
$ 1.92万
依托单位：
Kyorin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570821/
关键词：
Th1 cell Th2 cell CLA E-selectin ligand CCR-4 fucosyltransferase VII IL-4 IL-12 Th1 CD4T細胞 T細胞分化 Eセレクチン・リガンド fucosyltransferase VII アトピー性皮膚炎

项目摘要

We differentiated naive CD4^+T cells into Th1 or Th2 cells, then examined the expression of E-selectin ligand(ESL), fucosyltransferase VII(FucT-VII), and CCR-4 on those cells in a type-1 or type-2 cytokine environment, using flow cytometry, to determine what sort of cytokine environment is appropriate for the most efficient migration of Th1 cells or Th2 cells to the skin.1. Th1 cells showed intense expression of CLA, ESL, and FucT-VII, but not CCR-4 in the presence of IL-12. Th1 cells stimulated by IL-4 did not reveal the expression of CLA, but ESL and CCR-4, the latter being regarded to be specific of Th2 cells.2. Th2 cells expressed CCR-4 in the presence of IL-12, as well as IL-4, whereas there was neither CLA expression nor constant expression of ESL and FucT-VII in the presence of IL-4. In the presence of IL-12, the expression of CLA, ESL, and FucT VII was elevated.3. We analyzed the influence of Th1(IL-12) or Th2(IL-4)-pattern milieu on nonpolarized Th cells(Th0). Th0 cells differentiated into the cells with intense expression of both ESL and CLA in Th1 milieu, showing strong abilities to migrate to the skin, while in Th2 milieu, differentiation into CLA^+ cells was not observed, although ESL expression was increased.4. Th1 cells showed the expression of CCR-4 in addition to constant expression of ESL when cytokine mileu shifted from Th1 to Th2, while Th2 cells revealed ESL expression in addition to constant expression of CCR-4 when cytokine milieu shifted from Th2 to Th1.These obsernvations suggest that Th1 and Th2 cells obtain an ability to migrate to the skin with an expression of ESL, FucT-Vll, and CCR-4 in the process of activation. In addition, it is likely that Th1 and Th2 cells express a each phenotype that can migrate to the skin most efficiently, when they are exposed to opposite-pattern cytokine environment.

我们将初始CD 4 ^+T细胞分化为Th 1或Th 2细胞，然后使用流式细胞术检测这些细胞在1型或2型细胞因子环境中E-选择素配体（ESL）、岩藻糖基转移酶VII（FucT-VII）和CCR-4的表达，以确定哪种细胞因子环境适合于Th 1细胞或Th 2细胞最有效地迁移到皮肤。IL-12存在时，Th 1细胞表现出CLA、ESL和FucT-VII的强烈表达，但不表达CCR-4。IL-4刺激的Th 1细胞不表达CLA，但表达ESL和CCR-4，后者被认为是Th 2细胞特异性的. Th 2细胞在IL-12和IL-4的存在下表达CCR-4，而在IL-4的存在下既没有CLA表达也没有ESL和FucT-VII的恒定表达。在IL-12存在下，CLA、ESL和FucT VII的表达升高.我们分析了Th 1（IL-12）或Th 2（IL-4）模式环境对非极化Th细胞（Th 0）的影响。Th 0细胞在Th 1环境中分化为ESL和CLA均强表达的细胞，表现出较强的向皮肤迁移的能力，而在Th 2环境中，虽然ESL表达增加，但未观察到分化为CLA^+细胞.当细胞因子环境从Th 1向Th 2转变时，Th 1细胞除了恒定表达ESL外还显示CCR-4的表达，而当细胞因子环境从Th 2向Th 1转变时，Th 2细胞除了恒定表达CCR-4外还显示ESL的表达。和CCR-4在活化过程中。此外，当Th 1和Th 2细胞暴露于相反模式的细胞因子环境时，它们可能表达可以最有效地迁移到皮肤的每种表型。