Relationship between Chromosome Aberration in G2 and M Phase Cells and Radio-sensitivity and Development of Its Clinical Application

点击翻译按钮获取中文摘要

We have studied the relationship between individual radiosensitivity and chromosome aberration using Fluorescence in situ hybridization (FISH). To achieve this purpose, homozygous ataxia telangiectasia cells and normal human fibroblast cells were used. Confluent cultures of normal fibroblast cells (AG1522, AG1523, AG1518) and fibroblast cells derived from an individual with A-T (GM02052, GM01823) were exposed to gamma-rays and allowed to repair at 37 ℃ for 24-hr. At 10 % survival doses, GM02052 cells were approximately 5 times more sensitive to γ-rays than AG1522 cells. For a given dose, GM02052 cells contained a much higher frequency of deletions and misrejoining than AG1522 cells. For both cell types, good correlation was found between the percentage of aberrant cells and cell survival. These results indicate that both repair deficiency and misrepair may be involved in A-T hyper radiosensitivity. Similar studies are underway using human lymphocytes.