Relationship between Chromosome Aberration in G2 and M Phase Cells and Radio-sensitivity and Development of Its Clinical Application

G2、M期细胞染色体畸变与放射敏感性的关系及其临床应用进展

• 批准号: 14570838
• 负责人: KAWATA Tetsuya
• 金额: $ 2.24万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570838/
• 关键词: FISH; Chromosomal aberration; Radiosensitivity; Radiotherapy

We have studied the relationship between individual radiosensitivity and chromosome aberration using Fluorescence in situ hybridization (FISH). To achieve this purpose, homozygous ataxia telangiectasia cells and normal human fibroblast cells were used. Confluent cultures of normal fibroblast cells (AG1522, AG1523, AG1518) and fibroblast cells derived from an individual with A-T (GM02052, GM01823) were exposed to gamma-rays and allowed to repair at 37 ℃ for 24-hr. At 10 % survival doses, GM02052 cells were approximately 5 times more sensitive to γ-rays than AG1522 cells. For a given dose, GM02052 cells contained a much higher frequency of deletions and misrejoining than AG1522 cells. For both cell types, good correlation was found between the percentage of aberrant cells and cell survival. These results indicate that both repair deficiency and misrepair may be involved in A-T hyper radiosensitivity. Similar studies are underway using human lymphocytes.

本文应用荧光原位杂交技术研究了个体辐射敏感性与染色体畸变的关系。为了实现这一目的，使用纯合共济失调毛细血管扩张细胞和正常人成纤维细胞。将正常成纤维细胞（AG 1522、AG 1523、AG 1518）和来自A-T个体的成纤维细胞（GM 02052、GM 01823）的融合培养物暴露于γ射线，并在37 ℃下修复24小时。在10%存活剂量下，GM 02052细胞对γ射线的敏感性约为AG 1522细胞的5倍。对于给定的剂量，GM 02052细胞比AG 1522细胞含有更高频率的缺失和错连。对于这两种细胞类型，发现畸变细胞的百分比和细胞存活率之间存在良好的相关性。这些结果表明，修复缺陷和修复错误可能参与了A-T超辐射敏感性。类似的研究正在使用人类淋巴细胞进行。

项目成果

TETSUYA KAWATA: "Induction of Chromatin Damage and Distribution of Isochromatid Breaks in Human Fibroblast Cells Exposed to Heavy Ions"J Rad Res. (in press).

TETSUYA KAWATA：“暴露于重离子的人成纤维细胞中染色质损伤的诱导和等染色单体断裂的分布”J Rad Res。

Kawata T, et al.: "Radiation Induced Chromosome Aberrations in Ataxia Telangiectasia Cell Line : high frequency of deletions and misrejoining detected by fluorescence in situ hybridization."Radiat Res. 159. 597-603 (2003)

Kawata T 等人：“共济失调毛细血管扩张细胞系中辐射诱导的染色体畸变：通过荧光原位杂交检测到高频率的缺失和错误重连。”Radiat Res。

Kawata T: "G2 chromatid damage and repair kinetics in normal human fibroblast cells exposed to low- or high-LET radiation."Cytogenet Genome Res. (In press). (2003)

Kawata T：“暴露于低或高 LET 辐射的正常人成纤维细胞中的 G2 染色单体损伤和修复动力学。”Cytogenet Genome Res。

Uno T, et al.: "Preoperative radiation therapy for carcinoma of the oropharynx : Prognostic factors for locoregional control and survival."In vivo. 17(2). 239-244 (2003)

Uno T 等人：“口咽癌的术前放射治疗：局部区域控制和生存的预后因素。”体内。

Kawata T: "Induction of chromatin damage and distribution of isochromatid breaks in human fbroblast cells exposed to heavy ions."J Radiat Res. 43 Suppl. 169-173 (2002)

Kawata T：“暴露于重离子的人成纤维细胞中染色质损伤的诱导和等染色单体断裂的分布。”J Radiat Res。