Multi-step hepatocarcinogenesis and neovascularization-Observation by vital microscopy

多步骤肝癌发生和新生血管形成——活体显微镜观察

• 批准号: 14570841
• 负责人: MATSUI Osamu
• 金额: $ 2.5万
• 依托单位: KANAZAWA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570841/
• 关键词: hepatocellular carcinoma; fluorescent vital microscopy; experimental liver cancer; borderline lesion; tumor angiogenesis; multistep carcinogensis; liver metastasis; microcirculation; 血管新生; 肝微小循環; 生体顕微鏡

To analyze the changes of the sinusoidal microcirculation surrounding a tumor, selective embolization of the terminal portal venule was made in mice livers during the in vivo observation of sinusoidal flow by fluorescent vital microscopy.To clarify the early stage neovascularization of the hepatic carcinoma, experimental liver metastases and hepatocellular carcinoma and its borderline lesion were made and their microcirculation was observed by fluorescent vital microscopy.In the very early stage of metastasis, the cancer cells were fed by the portal venules.However, in ccordance with the growing of the tumor, internal dilated blood sinusoids became prominent gradually, These sinusoids had abundant connection with the surrounding hepatic sinusoids and were considered to be originated from the hepatic sinusoidal capillarization.In addition, the emodynamics of metastatic liver cancers and hepatocellular carcinomas were analyzed using imaging methods in comparison with histology and prognosis of the tumors in clinical cases.

为分析肿瘤周围肝窦微循环的变化，在荧光活体显微镜下观察肝窦血流的同时，选择性栓塞小鼠肝门静脉末端，以明确肝癌早期新生血管的形成。用荧光活体显微镜观察实验性肝转移瘤和肝癌及其交界性病变的微循环，在转移的早期，癌细胞由门静脉滋养，但随着肿瘤的生长，内部扩张的血窦逐渐突出，血窦与周围肝窦有丰富的联系，认为是肝窦毛细血管化的产物。应用影像学方法对转移性肝癌和肝细胞癌的血流动力学进行分析，并与临床病例的组织学和预后进行比较。

项目成果

Miyayama S, Matsui O, et al.: "Transcatheter arterial chemoembolization for unresectable hepatocellular carcinoma fed by the cystic artery"Journal of Vascular Interventional Radiology. 14(9). 1155-1161 (2003)

Miyayama S，Matsui O，等人：“经导管动脉化疗栓塞治疗由胆囊动脉供血的不可切除的肝细胞癌”血管介入放射学杂志。

Terayama N, Matsui 0, et al.: "Focal sparing of fatty liver in segment II associated with aberrant left gastric vein."British Journal of Radiology. 77. 150-152 (2003)

Terayama N、Matsui 0 等人：“与异常左胃静脉相关的 II 段脂肪肝的局灶性保留。”英国放射学杂志。

Terayama N: "Peritumoral rim enhancement of liver metastasis : hemodynanics observed on single-level dynamic CT during hepatic arteriography and histopathologic correlation"Journal of Computer Assisted Tomography. 26(6). 975-980 (2002)

Terayama N：“肝转移瘤周围边缘增强：肝动脉造影期间单层动态 CT 观察到的血流动力学和组织病理学相关性”计算机辅助断层扫描杂志。

Gabata T, Matsui 0, et al.: "Cystic duct remnant carcinoma with widespread invasion along the extrahepatic bile duct wall : dynamic CT findings."Abdominal Imaging. 28. 79-82 (2003)

Gabata T、Matsui 0 等人：“沿肝外胆管壁广泛侵犯的囊性管残留癌：动态 CT 发现。”腹部影像学。

Gabata T, Mataui O, et al.: "Cystic duct remnant carcinoma with widespread invasion along the extrahepatic bile duct wall : dynamic CT findings"Abdominal Imaging. 28. 79-82 (2003)

Gabata T、Mataui O 等人：“沿肝外胆管壁广泛侵犯的囊性管残留癌：动态 CT 发现”腹部影像学。