The Stufy of the Effect of Hepatic Copper Content on Signal Intensity in Magnetic Reasonance Images

肝铜对肝脏磁共振成像信号强度的影响：分析肝内铜异常积聚的LEC大鼠和Wistar大鼠的肝脏信号强度，研究肝脏信号强度与肝内铜含量的相关性。在T1和T2加权图像上，LEC大鼠肝脏信号强度与Wistar大鼠无明显差异。我们得出结论，铜积累可能不是T1加权图像上高强度hcc的原因。铜及铜结合蛋白对肝脏CT衰减值的影响：分析铜及铜结合蛋白对大鼠模型CT衰减值的影响。本研究包括5只被认为是威尔逊病大鼠型的LEC大鼠和28只Wistar大鼠。Wistar大鼠分为4组。正常对照组5只。12只大鼠结扎胆总管，在32 ~ 60天内腹腔内灌注更多铜（氯化铜溶液，日剂量1.8 ~ 2.4mg铜/kg）。11只大鼠腹腔注射高剂量铜（硝酸三乙酸铜，日剂量4 mg铜/kg），持续21 ~ 120天。用原子吸收分光光度法测定肝脏铜和铁。对所有大鼠进行了肝脏非增强CT的衰减值检查，并与肝脏铁和铜含量以及orcein阳性颗粒的等级进行了比较，orcein阳性颗粒被认为是溶酶体中聚合甲基硫蛋白的对应物。肝铜与CT衰减值差异无统计学意义（相关系数=0.056,p=0.96）。肝铁含量与衰减值的差异有统计学意义（R=0.561, p=0.007）。而CT值与orcein阳性颗粒的分级有很强的正相关（Spearman相关系数Rho=0.755， Rho校正后为0.738,p=0.0001）。LEC大鼠肝脏CT值与正常对照大鼠无明显差异。我们认为肝脏的CT衰减值可因溶酶体中富含聚合甲基硫蛋白（铜结合蛋白）而升高，且与肝脏铜含量呈负相关。少

Effect of hepatic copper on signal intensity in magnetic resonance imaging of the liver : We analyzed the signal intensity of the liver of LEC rats, which have an abnormal copper accumulation in the liver, and of Wistar rats, and studied the correlation between signal intensity of the liver and hepatic copper content. The signal intensity of the liver of LEC rat dose not differ from that of Wistar rats on both T1 and T2 weighted images. We concluded that copper accumulation may not be responsible for the high intensity of HCCs on T1 weighted images.Effect of copper and copper binding protein on CT attenuation value of the liver : We analyzed the effect of copper and copper binding protein on CT attenuation value in a rat model. Five Long-Evan's-cinnamon(LEC)rats, which are thought to be rat form of Wilson's disease, and 28 Wistar rats were included in this study. Wistar rats were divided into 4 groups. Five rats were normal control. In 12 rats, the common bile duct was ligated and addi … More tional copper(cupric chloride solution, daily dose of 1.8-2.4mg copper/kg)was intraperitoneally overloaded in 7 during 32-60 days. High dose copper(cupric nitrilotriacetate, daily dose of 4 mg copper/kg)was given intraperitoneally in 11 rats during 21-120 days. Hepatic copper and iron were measured by atomic absorption spectrophotometry. CT attenuation values at non-enhanced CT of the liver were examined in all rats and compare with hepatic iron and copper content, and grade of orcein positive granules that is thought to be a counter part of polymerized methalothionein in lysosome. The difference hepatic copper and attenuation value at CT was not statistically significant(correlation coefficient=0.056, p=0.96). The difference between hepatic iron content and attenuation value was statistically significant(R=0.561, p=0.007). However, CT value has a strong positive correlation with the grade of orcein positive granules(Spearman's correlation coefficient Rho=0.755, Rho corrected for ties, 0.738, p=0.0001). CT value of the liver of LEC rats did not differ from that of normal control rats. We concluded that CT attenuation value of the liver can be elevated by rich polymerized methalothionein(copper binding protein)in lysosome and has a negative correlation with hepatic copper content. Less