Analysis on the thymic epithelial cells in the thymus from the rats which were irradiated

辐照大鼠胸腺上皮细胞的分析

• 批准号: 14570861
• 负责人: FUKUMOTO Tetsuo
• 金额: $ 2.18万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570861/
• 关键词: thymic epithelial cells; thymus; irradiation; immnohistochemistry; RT-PCR; cytokines; CD4, CD8; MHC; 胸腺細胞; フローサイトメトリー

Rats were irradiated at the dotes of 6Gy or 8Gy. Thymus weights reduced after irradiation and recovered rapidly within 2 to 3 days after irradiation in the case of 6Gy irradiation, while in the 8Gy irradiation thymus recovery were retarded and started to recover on day 7 to 11 after irradiation. Subsequent recovery was up to 65% of control rat thymus. The mechanisms responsible for abrupt thymus weight were analysed immunohistochemically and by RT-PCR analysis on several cytokines, suggesting cytokine changes were intimately related to the thymic recovery

分别以6 Gy和8 Gy剂量照射大鼠。6 Gy照射后胸腺重量下降，在照射后2 ~ 3d内迅速恢复，8 Gy照射后胸腺重量恢复缓慢，在照射后7 ~ 11 d开始恢复。随后的回收率高达对照大鼠胸腺的65%。通过免疫组化和几种细胞因子的RT-PCR分析，对胸腺重量突变的机制进行了分析，表明细胞因子的变化与胸腺的恢复密切相关

项目成果

Nobuko, Tokuda: "MHC class II antigen expression was different from MHC class I antigen expression in irradiated and recovering rat thymus"Acta.Histochem.Cytochem.. 35(2). 101-105 (2002)

Nobuko, Tokuda：“受辐射和恢复的大鼠胸腺中 MHC II 类抗原表达与 MHC I 类抗原表达不同”Acta.Histochem.Cytochem.. 35(2)。

Nobuko, Tokuda: "Expression of PAC1 receptor in rat thymus after irradiation"Reg.Peptides. 115. 57 (2003)

Nobuko, Tokuda：“辐射后大鼠胸腺中 PAC1 受体的表达”Reg.Peptides。

Yasuhiro Adachi: "International symposium on biological effects of low dose radiation : proceedings"(2002)

安达靖弘：“低剂量辐射生物效应国际研讨会：会议记录”（2002）

Yamini, Arudchelvan: "Spatial relation between major histocompatibility complex-restricted antigen receptor-bearing thymocytes and subtypes of thymic epithelial cells"Anat.Rec.. 267. (2002)

Yamini，Arudchelvan：“主要组织相容性复合物限制性抗原受体承载胸腺细胞与胸腺上皮细胞亚型之间的空间关系”Anat.Rec.. 267。（2002）

Miyoshi T.: "Theromoluminescence of X-ray irradiated CdS-doped glass"Jpn.J.Appl.Phys.. 43. 1427-1428 (2004)

Miyoshi T.：“X射线照射的CdS掺杂玻璃的热致发光”Jpn.J.Appl.Phys.. 43. 1427-1428 (2004)