Cellular dynamics in the movement of hematopoietic foci

造血灶运动的细胞动力学

• 批准号: 07670017
• 负责人: FUKUMOTO Tetsuo
• 金额: $ 1.47万
• 依托单位: Yamaguchi Univeristy
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670017/
• 关键词: fetal liver; monoclonal antibody; immunohistochemistry; regenerating thymus; T cell maturation; thymic epithelial cells; blood vessels; 造血; Flow cytofluormetry; 造血巣の移動; ラット; 胎仔肝; BrdU; Flow cytofluorometry; 胸腺

We have analyzed fetal rat liver to study the mechanism responsible for the movement of hematopoiesis : In mammals, ontogenically hematopoietic foci move from yolk sac to fetal liver and eventually bone marrow becomes a predominant site for the hematopoiesis in adult. It is not know why hematopoiesis moves. In order to analyze the changes in fetal liver we have raised monoclonal antibodies and obtained UB11 and UB12 which label most cells in fetal liver hematopoietic cells and about 30% of adult bone marrow cells (Fujikura et al., 1990). And we used these to analyze the changes of hematopoiesis in fetal liver.In this study, we could get three other monoclonal antibodies to fetal liver cells (UB18, HAM10 and HAM11). UB18 labelled a few percent of fetal liver hematopoietic cells and adult bone marrow cells. The nature of the molecules which UB18 recognises are not known. HAM10 and HAM11 recognise some molecules in mitochondria and endoplasmic reticulum of fetal hepatocytes. The strong ap … More pearance of HAM10 antigen was observed when hematopoiesis are extensive in fetal liver, while HAM11 antigen was dominant when hematopoiesis started to disappear. Thus, we are on the way to study the relationship between the antigen expression changes of HAM10 and HAM11 and hematopoiesis (Lovely et al., 1996).We have also analyzed the regenerating thymus after irradiation, as the thymus reduces its size and function of T cell production after receiving 6Gy irradiation, while it recovers quite quickly. For example, on day 5 after irradiation thymocytes number increases 10 times of that of day 3, and on day cortico-meduallary boundary becomes obvious. Susequently, we considered that after irradiation and diminution in number of thymocytes, some quite potent abrupt supporting stromal function may work in thymic tissue. To study the changes in regenerating thymus, we analyzed the vascular changes of the thymus injecting Indian ink into the thymus to visualise the vascular structures. Results suggest that vascular structures were important for the abrupt thymic regeneration. We also analyzed thymic epithelial cells immunohistochemically using antibodies to thymic factors as FTS.Results suggest that the intensive staining of FTS was observed during the time of recovery. These data may support the adrupt proliferation of thymocytes in regenerating thymus. Less

我们分析了胎鼠肝脏来研究负责造血运动的机制：在哺乳动物中，个体发生的造血灶从卵黄囊移动到胎肝，最终骨髓成为成人造血的主要部位。不知道为什么造血会动。为了分析胎肝中的变化，我们提出了单克隆抗体并获得了UB 11和UB 12，它们标记了胎肝造血细胞中的大多数细胞和约30%的成人骨髓细胞（Fujikura等人，1990年）。本研究还获得了另外三种抗胎肝细胞的单克隆抗体（UB 18、HAM 10和HAM 11）。UB 18标记了几个百分比的胎肝造血细胞和成人骨髓细胞。UB 18识别的分子的性质尚不清楚。HAM 10和HAM 11识别胎肝细胞线粒体和内质网中的一些分子。强大的AP ...更多信息 当胎肝造血广泛时，HAM 10抗原占优势，当造血开始消失时，HAM 11抗原占优势。因此，我们正在研究HAM 10和HAM 11的抗原表达变化与造血之间的关系（Lovely等人，1996）。我们还分析了辐射后再生的胸腺，因为胸腺在接受6 Gy辐射后减小了其大小和T细胞产生的功能，但它恢复得相当快。如照射后第5天胸腺细胞数比第3天增加10倍，第5天皮质-髓质分界明显。因此，我们认为，在辐射和胸腺细胞数量减少后，胸腺组织中可能存在某些非常强有力的突然支持基质功能。为了研究再生胸腺的变化，我们分析了胸腺的血管变化，将印度墨水注射到胸腺中以使血管结构可视化。结果表明，血管结构是重要的突然胸腺再生。我们还用胸腺因子的抗体作为FTS对胸腺上皮细胞进行了免疫组化分析，结果表明在恢复期观察到FTS的强烈染色。这些结果可能支持再生胸腺中胸腺细胞的异常增殖。少

项目成果

福本哲夫: "「人体の世界」-免疫臓器と造血臓器のはなし" 日本解剖学会・風人社(印刷中), (1996)

Tetsuo Fukumoto：“人体的世界 - 免疫器官和造血器官的故事”日本解剖学会/Fujinsha（印刷中），（1996）

Li X.et al.: "Recognition of a special membrane antigen of squamous cell carcinoma in rats with a monoclonal antibody UB23" Tumor Biol.18. 350-355 (1997)

Li X.et al.：“用单克隆抗体UB23识别大鼠鳞状细胞癌的特殊膜抗原”Tumor Biol.18。

Fukumoto T.,: "Precise analyses of the strtucture of the thymus for establishing details of the mechanisms underlying thymocyte proliferation and maturation." Arch. Histol. Cytol.60. 1-8 (1997)

Fukumoto T.：“对胸腺结构进行精确分析，以确定胸腺细胞增殖和成熟机制的细节。”

T.Fukumoto: "Precise analyses on the structure of the thymus may establish details of the mechanisms underlying thymocyte proliferation and maturation." Arch.Histol.Cytol.(in press).

T.Fukumoto：“对胸腺结构的精确分析可以确定胸腺细胞增殖和成熟机制的细节。”

Ohba Y., et al.: "Analysis of allogenic lymphocytes in rat thymys following sublethal irradiation." Histol. Histopath.12. 337-342 (1997)

Ohba Y. 等人：“亚致死照射后大鼠胸腺中同种异体淋巴细胞的分析”。