Cellular dynamics in the movement of hematopoietic foci

我们分析了胎鼠肝脏来研究造血运动的机制：在哺乳动物中，造血病灶从卵黄囊转移到胎肝，最终骨髓成为成人造血的主要部位。目前尚不清楚造血运动的原因。为了分析胎儿肝脏的变化，我们培养了单克隆抗体，获得了UB11和UB12，它们标记了大多数胎儿肝脏造血细胞和约30%的成人骨髓细胞（Fujikura etal ., 1990）。我们用这些来分析胎儿肝脏造血功能的变化。在本研究中，我们可以获得另外三种针对胎儿肝细胞的单克隆抗体（UB18， HAM10和HAM11）。UB18标记了百分之几的胎儿肝脏造血细胞和成人骨髓细胞。UB18识别的分子的性质尚不清楚。HAM10和HAM11识别胎儿肝细胞线粒体和内质网中的一些分子。当胎儿肝脏造血广泛时，HAM10抗原较多出现，而当造血开始消失时，HAM11抗原占优势。因此，我们正在研究HAM10和HAM11抗原表达变化与造血之间的关系（Lovely et al., 1996）。我们还分析了辐照后胸腺的再生情况，由于胸腺在接受6Gy辐照后体积减小，T细胞生产功能下降，但恢复速度较快。例如，照射后第5天胸腺细胞数量比第3天增加10倍，第1天皮质-髓质边界变得明显。因此，我们认为在胸腺细胞辐照和数量减少后，胸腺组织中可能会出现一些非常强大的突然支持基质功能。为了研究再生胸腺的变化，我们对胸腺血管的变化进行了分析，并在胸腺内注射了印度墨水，以显示血管结构。结果表明，血管结构在胸腺突变再生中起重要作用。我们还使用胸腺因子抗体作为FTS免疫组织化学方法分析了胸腺上皮细胞。结果显示，在恢复过程中，FTS呈强烈染色。这些数据可能支持胸腺细胞在再生胸腺中的破坏增殖。少

We have analyzed fetal rat liver to study the mechanism responsible for the movement of hematopoiesis : In mammals, ontogenically hematopoietic foci move from yolk sac to fetal liver and eventually bone marrow becomes a predominant site for the hematopoiesis in adult. It is not know why hematopoiesis moves. In order to analyze the changes in fetal liver we have raised monoclonal antibodies and obtained UB11 and UB12 which label most cells in fetal liver hematopoietic cells and about 30% of adult bone marrow cells (Fujikura et al., 1990). And we used these to analyze the changes of hematopoiesis in fetal liver.In this study, we could get three other monoclonal antibodies to fetal liver cells (UB18, HAM10 and HAM11). UB18 labelled a few percent of fetal liver hematopoietic cells and adult bone marrow cells. The nature of the molecules which UB18 recognises are not known. HAM10 and HAM11 recognise some molecules in mitochondria and endoplasmic reticulum of fetal hepatocytes. The strong ap … More pearance of HAM10 antigen was observed when hematopoiesis are extensive in fetal liver, while HAM11 antigen was dominant when hematopoiesis started to disappear. Thus, we are on the way to study the relationship between the antigen expression changes of HAM10 and HAM11 and hematopoiesis (Lovely et al., 1996).We have also analyzed the regenerating thymus after irradiation, as the thymus reduces its size and function of T cell production after receiving 6Gy irradiation, while it recovers quite quickly. For example, on day 5 after irradiation thymocytes number increases 10 times of that of day 3, and on day cortico-meduallary boundary becomes obvious. Susequently, we considered that after irradiation and diminution in number of thymocytes, some quite potent abrupt supporting stromal function may work in thymic tissue. To study the changes in regenerating thymus, we analyzed the vascular changes of the thymus injecting Indian ink into the thymus to visualise the vascular structures. Results suggest that vascular structures were important for the abrupt thymic regeneration. We also analyzed thymic epithelial cells immunohistochemically using antibodies to thymic factors as FTS.Results suggest that the intensive staining of FTS was observed during the time of recovery. These data may support the adrupt proliferation of thymocytes in regenerating thymus. Less