Analysis of paihophysiology of thiombosis through the adhesion molecules : the relationship between heterogeneities in lupus anticoagulant antibodies and release of adhesion molecules in vitro.

通过粘附分子分析血栓形成的病理生理学:狼疮抗凝抗体的异质性与体外粘附分子释放之间的关系。

基本信息

  • 批准号:
    14570970
  • 负责人:
  • 金额:
    $ 2.3万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2002
  • 资助国家:
    日本
  • 起止时间:
    2002 至 2003
  • 项目状态:
    已结题

项目摘要

Antiphospholipid syndrome(APS) is an autoimmune disease characterized by recurrent thromboses and pregnant morbidity, although pathophysiologies of these clinical features have not been clarified yet.Recent papers reported that adhesion-molecules including intercellular adhesion molecule-1(ICAM-1) and P-selectin play important roles in thrombosis of patients with APS.We also reported that anti-prothrombin antibodies(anti-PT), which is one of major antiphospholipid antibodies (aPL), have 4 subtype of anti-PT and that anti-PT #1, which bound to human prothrombin only in the presence of anionic phospholipid and calcium ions, was significantly associated with thromboses. From these findings, we evaluate the association of the release of adhesion molecules including VCAM-1, ICAM-1 and E-selectin by human umbilical vein endothelial cells(HUVEC) and these subtypes of anti-PT.Medium levels of VCAM-1, ICAM-1, and E-selectin were significantly higher in addition of anti-PT#1 compared with those of normal IgG.(p<0.001, <0.001, and <0.005, respectively).Medium ICAM-1 levels but not VCAM-1 and E-selectin significantly increased on anti-PT#2(p<0.05)compared with normal IgG.Those medium levels did not increased on anti-PT#3 or #4. These results suggested that heterogeneities of anti-PT occurred to different releases of adhesion molecules and that those differentiations may be associated with the different activations of endothel, monocytes, and platelets, which induced to heterogeneity of clinical features on APS.
抗磷脂综合征(Antiphospholipid syndrome,APS)是一种以反复血栓形成和妊娠并发症为特征的自身免疫性疾病,其病理生理机制尚不清楚,近年来的研究表明,细胞间粘附分子-1(intercellular adhesion molecule-1,ICAM-1)和P-选择素(P-selectin)在APS患者血栓形成中起重要作用,抗凝血酶原抗体(anti-prothrombin antibodies,PT),其是主要抗磷脂抗体(aPL)之一,具有4种抗PT亚型,且抗PT #1仅在阴离子磷脂和钙离子存在下与人凝血酶原结合,与血栓形成显著相关。从这些发现中,我们评估了粘附分子,包括VCAM-1,ICAM-1和E-选择素的人脐静脉内皮细胞(HUVEC)和这些亚型的抗PT的释放的关联。VCAM-1,ICAM-1,和E-选择素的中水平显着高于除了抗PT #1与正常IgG相比。(分别为p<0.001、p <0.001和p <0.005)。与正常IgG相比,在抗PT #2上,中等ICAM-1水平显著增加,而VCAM-1和E-选择素水平不显著增加(p<0.05)。这些结果提示,抗PT抗体的异质性与粘附分子的释放不同有关,这种差异可能与内皮细胞、单核细胞和血小板的不同活化有关,从而导致APS临床特征的异质性。

项目成果

期刊论文数量(112)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
山崎雅英, 石山謙, 飯田恵, 斉藤祐希, 奥村廣和, 朝倉英策, 中尾眞二: "播種性血管内凝個(DIC)を併発した急性骨髄性白血病妊婦に対するメシル酸ナファモスタットの使用経験"医薬の門. 43(3). 492-495 (2003)
Masahide Yamazaki、Ken Ishiyama、Megumi Iida、Yuki Saito、Hirokazu Okumura、Eisaku Asakura、Shinji Nakao:“甲磺酸萘莫司他在患有急性髓性白血病并发弥散性血管内凝血 (DIC) 的孕妇中的使用经验”Pharmaceutical Gate 43( 3)492-495(2003)。
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Asakura H, Yamazaki M, Morishita E, et al.: "Beneficial effect of JTV-803, a new synthetic inhibitor of activated factor X, against both lipopolysaccharide-induced and tissue factor-induced disseminated intravascular coagulation in rat models"Blood Coagul
Asakura H、Yamazaki M、Morishita E 等人:“JTV-803(一种新型合成的活化因子 X 抑制剂)对大鼠模型中脂多糖诱导和组织因子诱导的弥散性血管内凝血的有益作用”
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山崎雅英: "血液疾患臨床ハンドブツク(中村 忍、中尾 眞二 編著)"中外医学社. 251-265 (2002)
Masahide Yamazaki:“血液疾病临床手册(中村忍和中尾真司编辑)”中外医学社 251-265(2002)。
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    0
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Asakura H, Yamazaki M, et al.(全14名, 9番目): "Beneficial effect of JTV-803, a new synthetic inhibitor of activated factor X, against both lipopolysaccharide-induced and tissue factor-induced disseminated intravascular coagulation in rat models."Blood Coagula
Asakura H、Yamazaki M 等人(共 14 人,第 9 名):“JTV-803(一种新型合成的活化因子 X 抑制剂)对脂多糖诱导和组织因子诱导的大鼠弥散性血管内凝血的有益作用模型。“血液凝固
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Sano Y, Yamazaki M, et al.: "Change in plasma levels of vasoactive substances in rat DIC models."Jpn J Thromb Hemost. 13(6). 485-492 (2002)
Sano Y、Yamazaki M 等人:“大鼠 DIC 模型中血管活性物质血浆水平的变化。”Jpn J Thromb Hemost。
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YAMAZAKI Masahide其他文献

YAMAZAKI Masahide的其他文献

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{{ truncateString('YAMAZAKI Masahide', 18)}}的其他基金

Development of novel therapy which targets B lymphocytes against antiphospholipid syndrome
开发针对 B 淋巴细胞的抗磷脂综合征新疗法
  • 批准号:
    19591101
  • 财政年份:
    2007
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Pathophysiology of adhesion molecules for induction of antiphospholipid antibodies and occurrence of thrombosis in antiphospholipid syndrome: Analysis of those mechanisms using adhesion-molecules knock out mice.
粘附分子诱导抗磷脂抗体和抗磷脂综合征中血栓形成的病理生理学:使用粘附分子敲除小鼠分析这些机制。
  • 批准号:
    17590985
  • 财政年份:
    2005
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
International Aspects of Local Communities : Legal Analysis
当地社区的国际方面:法律分析
  • 批准号:
    01410015
  • 财政年份:
    1989
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (A)

相似海外基金

Standardization of the measuring method for lupus anticoagulant (LA)
狼疮抗凝物(LA)测量方法标准化
  • 批准号:
    23590684
  • 财政年份:
    2011
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
LUPUS ANTICOAGULANT INDUCED ENDOTHELIAL INJURY
狼疮抗凝剂引起的内皮损伤
  • 批准号:
    3050542
  • 财政年份:
    1988
  • 资助金额:
    $ 2.3万
  • 项目类别:
LUPUS ANTICOAGULANT INDUCED ENDOTHELIAL INJURY
狼疮抗凝剂引起的内皮损伤
  • 批准号:
    3050541
  • 财政年份:
    1987
  • 资助金额:
    $ 2.3万
  • 项目类别:
TREATMENT OF LUPUS ANTICOAGULANT IN PREGNANCY
妊娠期狼疮抗凝剂的治疗
  • 批准号:
    3320646
  • 财政年份:
    1987
  • 资助金额:
    $ 2.3万
  • 项目类别:
TREATMENT OF LUPUS ANTICOAGULANT IN PREGNANCY
妊娠期狼疮抗凝剂的治疗
  • 批准号:
    3320644
  • 财政年份:
    1987
  • 资助金额:
    $ 2.3万
  • 项目类别:
LUPUS ANTICOAGULANT AND RECURRENT PREGNANCY LOSS
狼疮抗凝剂与复发性流产
  • 批准号:
    3320405
  • 财政年份:
    1986
  • 资助金额:
    $ 2.3万
  • 项目类别:
LUPUS ANTICOAGULANT AND RECURRENT PREGNANCY LOSS
狼疮抗凝剂与复发性流产
  • 批准号:
    3320406
  • 财政年份:
    1986
  • 资助金额:
    $ 2.3万
  • 项目类别:
LUPUS ANTICOAGULANT AND RECURRENT PREGNANCY LOSS
狼疮抗凝剂与复发性流产
  • 批准号:
    3320402
  • 财政年份:
    1986
  • 资助金额:
    $ 2.3万
  • 项目类别:
LUPUS ANTICOAGULANT IN PREGNANCY
妊娠期狼疮抗凝剂
  • 批准号:
    3956565
  • 财政年份:
  • 资助金额:
    $ 2.3万
  • 项目类别:
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