LUPUS ANTICOAGULANT AND RECURRENT PREGNANCY LOSS

狼疮抗凝剂(LAC)是一种自身抗体，与 复发性早孕流产和中期胎儿死亡， 使用抗凝剂的妇女中80%或更多的妊娠结局为胎儿 浪费。在妊娠中期以后进展、严重或 早期子痫前期和胎儿生长迟缓是常见的。Lac是 通常通过其对磷脂依赖的延长效应来检测 凝血，该抗体已被鉴定为抗磷脂。 LAC的病理生理效应被认为是由于抑制了 在体外观察到的血管前列环素的产生 抗体的存在。我们建议从两个方面研究本地接驳费。第一, 我们将使用以下方法评估LAC的特异性抗原-抗体相互作用 这是我们开发的一种免疫分析方法。我们的初步结果 提示该抗原为磷脂、磷脂酰丝氨酸。 其次，我们将使用前列环素来研究LAC的作用机制 世代试验和小鼠、兔的繁殖模型。动物们 会被各种磷脂制剂主动免疫或 用纯化的人LAC制剂或人或小鼠被动免疫 并对其繁殖性能进行了评价。

Lupus anticoagulant (LAC) is an autoantibody that is associated with recurrent first-trimester abortion and second-trimester fetal death, with 80% or more of pregnancies in women with the anticoagulant ending in fetal wastage. In pregnancies that progress beyond mid-gestation, severe or early preeclampsia and fetal growth retardation are common. LAC is commonly detected by its prolongation-effect on phospholipid-dependent coagulation, and the antibody has been identified as an anti-phospholipid. The pathophysiologic effect of LAC has been attributed to the inhibition of vascular prostacyclin production that has been observed in vitro in the presence of the antibody. We propose to study two aspects of LAC. First, we will evaluate the specific antigen-antibody interactions for LAC using an immunologic assay that we have developed. Our preliminary results suggest that the antigen is the phospholipid, phosphatidylserine. Secondly, we will study the mechanism of LAC using a prostacyclin generation assay and mouse and rabbit models of reproduction. The animals will be actively immunized with various phospholipid preparations or passively immunized with purified human LAC preparations or human or mouse monoclonal LAC, and their reproductive performance evaluated.