Effects of Maternal Deprivation on Brain Development in Rats

母亲剥夺对大鼠大脑发育的影响

• 批准号: 14571050
• 负责人: MIKI Takanori
• 金额: $ 2.56万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571050/
• 关键词: maternal deprivation; hippocampal neurons; perinatal stress; brain development; neurotrophic factors; BDNF mRNA; Real-time PCR; stereology; 母性剥奪; ラット海馬; 定量解析; ストレス; 成人病素因; 周生期ストレス; 海馬; 脳発達; 可塑性; 母子間相互作用

We have established the experimental animal (rat) model of maternal deprivation (MD) in human by separating pups from mothers for 3 h/day during postnatal days (PND) 10-15.Effects on body and pituitary weightsThe body weight of MD rats showed no significant difference at PND16, 20 and 30, whereas at PND60 it was high compared to age-matched control. On the other hand, pituitary weight was low at any time points of observation.Effects on brain weightThe brain weight of MD rats showed no significant difference at any time points of observation.Corticosterone concentrationDuring the separation period, blood corticosterone concentration of MD rats was significantly higher (51ng/dL) than that of controls (32ng/dL). After separation, it was declined to 40 ng/dL.Stereological analysis (physical disector)There were no significant difference in total number of pyramidal cells in CA1-3 regions, and granule and hilus neurons in the dentate gyrus of MD rats compared to controls. Additionally, numerical density and the regional volume of MD rats showed no differences compared to controls.Real-time PCR analysis of BDNF mRNAMD rats had less BDNF mRNA in the hippocampus at PND16, whereas at PND 30 and 60 they had much BDNF mRNA than age-matched control animals.

本研究采用产后10- 15天（PND）将母鼠与母鼠分离3 h/d的方法建立了人类母亲剥夺（MD）的实验动物（大鼠）模型。MD对大鼠体重和脑垂体重量的影响MD大鼠体重在PND 16、20和30天与同龄对照组相比无显著性差异，而在PND 60天与同龄对照组相比明显增加。脑重量的影响MD大鼠脑重量在各观察时间点均无显著性差异。皮质酮浓度在分离期间，MD大鼠血皮质酮浓度（51 ng/dL）显著高于对照组（32 ng/dL）。体视学分析（物理解剖）与对照组相比，MD大鼠海马CA 1 -3区锥体细胞总数、齿状回颗粒和门神经元总数无显著性差异。此外，数密度和区域体积的MD大鼠显示没有显着差异相比，controls.Real-time PCR分析的BDNF mRNAMD大鼠有较少的BDNF mRNA在PND 16海马，而在PND 30和60他们有大量的BDNF mRNA比年龄匹配的对照动物。

项目成果

Identification of a novel variant of the human NR2B gene promoter region and its possible association with schizophrenia

• DOI: 10.1038/sj.mp.4001152
• 发表时间: 2002-01-01
• 期刊: MOLECULAR PSYCHIATRY
• 影响因子: 11
• 作者: Miyatake, R;Furukawa, A;Suwaki, H
• 通讯作者: Suwaki, H

The effects of alcohol exposure during early life on neuron numbers in the rat hippocampus : I)hilus neurons and granule cells.

生命早期接触酒精对大鼠海马神经元数量的影响：I)门神经元和颗粒细胞。

• 发表时间: 2003
• 期刊: Hippocampus 13
• 作者: Sakurai et al.;Miki et al.
• 通讯作者: Miki et al.

Relationship between cerebral interstitial levels of amino acids and phosphorylation potential during secondary energy failure in hypoxic-ischemic newborn pialets

缺氧缺血新生儿继发性能量衰竭时脑间质氨基酸水平与磷酸化电位的关系

• 发表时间: 2004
• 期刊: Pediatr Res 55
• 作者: Miki T;Satriotomo I;Li H-P;Matsumoto Y;He G;Yokoyam T;Lee K-Y;Bedi KS;Takeuchi Y;Takanori Miki et al.;Takashi Kusaka et al.
• 通讯作者: Takashi Kusaka et al.

Application of the physical disector to central nervous system : estimation of the total number of neurons in the subdivisions of the rat hippocampus.

物理解剖器在中枢神经系统中的应用：估计大鼠海马分区中的神经元总数。

• 发表时间: 2005
• 期刊: Anat Sci Int 80
• 作者: Miki et al.

Kindling of the ventral tegmental area induces supersensitivity in the central dopamine system.

腹侧被盖区的点燃会引起中枢多巴胺系统的超敏感性。

• 发表时间: 2004
• 期刊: Brain Res 1003
• 作者: Watanabe et al.