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METABOLIC CHANGES IN THE CEREBROSPINAL FLUID OF PATIENTS WITH SPINAL DEGENERATIVE DISOEDERS

脊柱退行性疾病患者脑脊液的代谢变化

基本信息

批准号：
14571377
负责人：
NAGASHIMA Hideki
金额：
$ 1.02万
依托单位：
NATIONAL UNIVERSITY CORPORATION TOTTORI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

The cerebrospinal fluid (CSF) samples were obtained by lumbar puncture from patients with cervical myelopathy (Group M), lumbar radiculopathy (Group R) prior to myelography, and 10 volunteers (control). Using high resolution nuclear magnetic resonance (NMR) spectroscopy, lactate, alanine, acetate, glutamate, pyruvate, and citrate concentrations in CSF were measured. Also the concentrations of interleukin 1 beta (IL-1-beta), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha) in CSF were measured. In Groups M and R, we evaluated the differences in the above-mentioned metabolites and cytokines with in control. Moreover, the relationships between each metabolite or cytokine and the severity of these disorders or symptom duration were evaluated. The severity of diseases was scored using Japanese Orthopedic Association (JOA) score for cervical myelopathy in Group M or JOA score for lumbar disorders in Group B. In Group B we also evaluated the relationship between each metabolite or cytokine and the duration of the relief from radiculopathy after a root block.In Group M, none of cytokines or metabolites was not significantly difference compared to control. Each metabolite or cytokine demonstrated no significant relationship with the symptom duration or JOA score. In Group R, IL-6 was significantly higher, and citrate was significantly lower than in control. Lactate, glutamate, and citrate had significantly positive relationship with symptom duration. TNF-alpha demonstrated significantly positive relationship and pyruvate had significantly negative relationship with duration of the relief from radiculopathy after root block.Our data suggested increased aerobic metabolism was shown in the patient with lumbar radiculopathy.

脑脊液（CSF）样本通过腰椎穿刺从脊髓造影前的颈椎病（M组）、腰椎神经根病（R组）患者和10名志愿者（对照组）中获得。使用高分辨率核磁共振（NMR）光谱法，测量CSF中的乳酸盐、丙氨酸盐、乙酸盐、谷氨酸盐、丙酮酸盐和柠檬酸盐浓度。同时测定脑脊液中白细胞介素1 β（IL-1-β）、白细胞介素6（IL-6）、肿瘤坏死因子α（TNF-α）的浓度。在M组和R组中，我们评估了上述代谢产物和细胞因子与对照组的差异。此外，还评估了每种代谢物或细胞因子与这些疾病的严重程度或症状持续时间之间的关系。对M组颈椎病采用日本骨科协会（乔亚）评分，对B组腰椎疾病采用乔亚评分。在B组中，我们还评估了每种代谢物或细胞因子与根阻滞后神经根病缓解时间的关系。在M组中，与对照组相比，细胞因子或代谢物均无显著差异。每种代谢物或细胞因子与症状持续时间或乔亚评分无显著相关性。在R组，IL-6显著高于对照组，而柠檬酸盐显著低于对照组。乳酸、谷氨酸和柠檬酸与症状持续时间呈显著正相关。TNF-α与神经根阻滞后症状缓解时间呈显著正相关，丙酮酸与神经根阻滞后症状缓解时间呈显著负相关，提示腰椎神经根病患者有氧代谢增强。