Neuronal activities of lumbar dorsal horn cells in rats model of lumbar radiculopathy

腰椎神经根病大鼠模型腰背角细胞的神经活性

• 批准号: 09671500
• 负责人: TAMI Toshikazu
• 金额: $ 1.86万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671500/
• 关键词: Neuropathy; Pain; Hypersensitivity; Hyperactivity

To investigate abnormal sensation such as hypesthesia, paresthesia, etc. observed in natient with peripheral nerve compression injury, we produced animal nerve compression model and observed the reactions of dorsal horn neuronal responses. Experiments were done in anesthetized male Sprague-Dawlev rats. Responses of wide dynamic range (WDR) cells (n=13) in the lumbar dorsal horn to cutaneous stimuli (brush, pressure, pinch) were recorded before and during nerve compression and after releasing of the compression. Depths of the neurons were 500-1200 mu m from the dorsal surface of the spinal cord. Nerve compression was produced by sciatic nerve applied small vessel clamp (120g of pinch force). The damp was attached 30 mm of duration. The results obtained so far, indicate that after the nerve compression, responses to the innocuous stimuli (brush) were decreased but responses to the noxious stimuli (pinch) were not affected. hive mm. after detachment of the nerve clamp, 11 neurons demonstrated increased brush responses temporarily.Present results suggest that in dorsal horn WDR neurons, responses to innocuous stimuli (A BETA )were more likely to he decreas by the nerve compression than those to noxious stimuli (Adelta/C).

为探讨周围神经卡压伤后感觉减退、感觉异常等异常感觉，我们制作了周围神经卡压动物模型，观察了脊髓背角神经元的反应。实验在麻醉的雄性Sprague-Dawlev大鼠中进行。宽动态范围（WDR）细胞（n=13）在腰椎背角皮肤刺激（刷，压力，捏）的反应被记录之前和期间的神经压迫和释放后的压缩。神经元的深度为500-1200 μ m。用小血管夹（120 g夹持力）夹持坐骨神经造成神经压迫。湿气附着30 mm的持续时间。到目前为止获得的结果表明，神经压迫后，对无害刺激（刷）的反应降低，但对有害刺激（捏）的反应不受影响。结果表明，在背角WDR神经元中，对无害刺激的反应（A β）比对伤害性刺激的反应（A δ/C）更容易因神经压迫而减弱。

项目成果

川崎 元敬: "急性坐骨神経圧迫モデルにおける腰髄部後角細胞の活動性の経時的変化" 日本整形外科学会雑誌. Vol.72,No.8. 1622 (1998)

Mototaka Kawasaki：“急性坐骨神经受压模型中腰脊髓背角细胞活动的时间变化”，日本骨科学会杂志，第 72 卷，第 1622 期（1998 年）。

Takahiro Ushida: "Neuronal Activities of Lumbar Dorsal Horn Cells in Rats with Sciatic Nerve Compression" Society for Neuroscience Abstracts. Vol.23. 559 (1997)

Takahiro Ushida：“坐骨神经受压大鼠腰背角细胞的神经活动”神经科学学会摘要。