Repair and replace the degenerative or defective cartilage using tissue engineering

利用组织工程修复和替换退化或有缺陷的软骨

• 批准号: 14571397
• 负责人: KITANO Toshio
• 金额: $ 2.11万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571397/
• 关键词: Repair of cartilage; Lubrication; Arthorplasty; DDS; 軟骨組織再生; 軟骨培養; 潤滑; 関節修復; 組織工学

Hyaluronic acid and/or Chondroitin sulfate capsulated in Lα-DPPC (dipalmitoylphosphatidylcholine) liposome could accelerate the repair of degenerative and/or defective articular cartilage. Using this Lα-DPPC drug delivery system (DDS), we investigate the lubrication between the disk shaped specimens from porcine knee joint. These results showed that Hyaluronic acid encapsulated in the cationic liposome for the lubricant decreased the coefficient of friction between cartilage specimens at low angular velocity. At low angular velocity, boundary lubrication mechanism is dominant, especially in the degenerative and/or defective articular. For the purpose of accelerating the repair of degenerative and/or defective articular cartilage, it is important to keep low friction on articulate cartilage surfaces during cartilage repair. This research result suggested the possibility of new treatment system of the arthorplasty, i.e., repairing the degenerative and/or defective articular cartilage using 1) the endoscopic surgery and 2) the incubation of the cartilage on site 3) without using artificial scaffold nor culture dish. This research develops the following research project (Project number : 18591672, Drug delivery system to improve the joint lubrication and to accelerate the regeneration of joint cartilage.

二棕榈酰磷脂酰胆碱（DPPC）脂质体包封的透明质酸和/或硫酸软骨素可促进退行性和/或缺损关节软骨的修复。利用Lα-DPPC给药系统（DDS），研究了猪膝关节盘状标本间的润滑情况。这些结果表明，包封在阳离子脂质体中的透明质酸作为润滑剂降低了软骨样品在低角速度下的摩擦系数。在低角速度下，边界润滑机制占主导地位，特别是在退行性和/或有缺陷的关节。为了加速退行性和/或有缺陷的关节软骨的修复，在软骨修复期间保持关节软骨表面上的低摩擦是重要的。本研究结果提示了关节成形术新治疗系统的可能性，修复退化和/或有缺陷的关节软骨，使用1）内窥镜手术和2）软骨的原位孵育3）而不使用人工支架或培养皿。本研究开展了以下研究项目（项目编号：18591672，改善关节润滑和加速关节软骨再生的药物输送系统。