The roles IGF of family in renal cell carcinoma

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It is reported that Insulin-like growth factor (IGF) system participates in growth and differentiation of both some normal cells and cancer cells. Generally, IGFs (IGF-I, IGF-II), type I and II IGF receptors, IGF-binding proteins (IGFBPs., and IGFBP proteases are contained in IGF system, and act as a mitogenic factor by combining IGF with type I and II IGF receptors or insulin-receptor. We-created gene profiling using the cDNA microarray, and we found that IGFBP3 and insulin receptor of IGF family highly expressed in renal cell carcinoma. We compared expression level of IGF family in the normal renal parenchyma with that in renal cell carcinama using RNase protein assay (RPA), and we estimated prognosis significance. As a result of inquiring by total of 13 genes, such as IGF1; 16F2, type I and II IGF receptors, H19 and ALS, insulin receptor, and IGFBP 1-6, strong expression was found in 100% of IGFBP3, 42% of insulin receptor, and 42% of IGFBP1 in 12 patients with renal cell Carcinoma. Furthermore, as a result of examining the expression level in the serum of such protein, IGFBP3 was intentionally high at the renal cell carcinoma patient. In the average of 17 months follow-up, a significant difference was not found as the prognosis factor, but further long-term follow-up was required. It was thought that measurement of IGF family was useful to diagnosis, and might become the target gene of renal cell carcinoma for gene therapy.