The interaction of cancer cell and stromal cell in urinary cancer-The approach of stromal therapy-

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In Micro array assay, we made a comparative study on bladder cancer cell line 5637 treated with or without hepatocyto growth factor(HGF), focus ing attention on toll like receptor 4(TLR4), Matrix metal loproteases-1(MMP-1) and MMP-10 of 26 molecule, more than 2-fold change(3down-regulation, 23up-regulation). We also assessed the TLR4, MMP-1 and MMP-10 involvement in the invasion and proliferation of bladder cancer.TLR4, MMP-1 and MMP-10 mRNA levels i n invasive bladder cancer cells treated with HGF were dose-dependently elevated in real-time PCR. The escalation of MMP-1and MMP-10 activity was inhibited by knockdown of TLR4 in 5637 cells treated with HGF.In conclusion, we speculate that the effect of HGF lead to enhance the ability of invasiveness by escalating the activity of MMP through the signal of TLR4-NFκB.