The interaction of cancer cell and stromal cell in urinary cancer-The approach of stromal therapy-

在微阵列实验中，我们对加肝细胞生长因子（HGF）和不加肝细胞生长因子（HGF）对膀胱癌细胞株5637的影响进行了比较研究，重点关注toll样受体4（TLR4）、基质金属蛋白酶-1（MMP-1）和MMP-10 26个分子的表达变化，变化幅度超过2倍（下调3个，上调23个）。我们还评估了TLR4、MMP-1和MMP-10参与膀胱癌的侵袭和增殖。real-time PCR结果显示，HGF处理的侵袭性膀胱癌细胞中TLR4、MMP-1和MMP-10 mRNA水平呈剂量依赖性升高。在HGF处理的5637个细胞中，通过敲低TLR4抑制mmp -1和MMP-10活性的升高。综上所述，我们推测HGF的作用可能是通过TLR4-NFκB信号使MMP活性升高，从而导致其侵袭能力增强。

In Micro array assay, we made a comparative study on bladder cancer cell line 5637 treated with or without hepatocyto growth factor(HGF), focus ing attention on toll like receptor 4(TLR4), Matrix metal loproteases-1(MMP-1) and MMP-10 of 26 molecule, more than 2-fold change(3down-regulation, 23up-regulation). We also assessed the TLR4, MMP-1 and MMP-10 involvement in the invasion and proliferation of bladder cancer.TLR4, MMP-1 and MMP-10 mRNA levels i n invasive bladder cancer cells treated with HGF were dose-dependently elevated in real-time PCR. The escalation of MMP-1and MMP-10 activity was inhibited by knockdown of TLR4 in 5637 cells treated with HGF.In conclusion, we speculate that the effect of HGF lead to enhance the ability of invasiveness by escalating the activity of MMP through the signal of TLR4-NFκB.