Development ofa novel therapeutic drug for treatment of uterine cervical incompetence

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We continued study about the effect of MU for hyaluronic acid synthesis in human cervical fibroblast this time. It was studied whether HA synthesis in the cell was inhibited under cytokine presence, which play an important role for cervical ripening and onset og labor, by MU addition.METHODSHuman cervical fibroblasts obtained at the time of an operation was cultured.It was added IL-1 β,IL-6 and IL-8(0.1〜50ng/ml) to a cultured medium.The medium was collected after it was divided a cultured cell more into a group of MU addition, a group of non-addition, and it was cultured for 48 hours. The amount of HA was measured by sandwitch binding protein assay method with HA binding protein.REAULTSThe amount of HA synthesized by human cervix fibroblasts increased in IL-8, an IL-6 addition group in comparison with a group of contorol(a cytokine additive-free group) significantly. However, the amount of HA was inhibited in less than 50% in all groups by addition of MU.CONCLUSIONThe finding that MU almost completely inhibited production of hyaluronic acid, which plays an important role in ripening of the cervix, suggested an inhibitory effect of MU on ripening of the cervix. Therefore, we propose that MU may be a novel therapeutic drug to treat cervical incompetence, and to prevent preterm delivery.