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Maternal Fatal cell traffic by DNA analysis and New approach for Autoinunune disorder

通过 DNA 分析进行母体致命细胞运输和治疗自身免疫性疾病的新方法

基本信息

批准号：
14571590
负责人：
YAMAGUCHI Nobuo
金额：
$ 2.11万
依托单位：
KANAZAWA MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571590/
关键词：
materno-fatal relation pregnancy MHC restriction CD4 T cell MHC auto immunity cell traffic GVH

项目摘要

We have previously reported that the immunization of pregnant mice with T-dependent antigens successfully induced suppression of the antigen-specific plaque-forming cell (PFC) response to the relevant antigens in the offspring. This suppression was not caused by the administered antigens, the antibodies produced by the pregnant mother or lactational transfer, but was dependent on the presence of the intact maternal T cells. It was major histocompatibility complex (MHC)-restricted manner tolerance, which continued for at least one-sixth of the marine life. Traditionally, the placenta acts as a natural barrier, not allowing the cells to pass through. However, the results presented strongly suggested that maternal T cells pass through the placenta and subsequently induce tolerance. In this present study, we attempted to substantiate the presence of maternal cells in the fetal circulation through the use of molecular techniques. We found that a highly polymorphic microsatellite sequence within the class II Eb gene of the H-2 complex is useful for the molecular detection of various H-2 alleles. DNA polymorphic analysis was used for tracking maternal H-2 alleles in the spleens of baby mice.According to our fundamental approaches, next we tried to prove the maternal cell trafficking in human system, after satisfactory organizing informed consents. We tried to picked up the HLA locus A, B, C, DR, DQ of mother and her children, aged 11 and 13 years old. But we have not yet obtained complete trafficking of mother cell that showed whole set of HLA locus from mother in her children. We have to increase the case and select the patient of autoimmune syndrome such as SLE etc.

我们以前曾报道，免疫孕鼠与T-依赖性抗原成功地诱导抑制抗原特异性空斑形成细胞（PFC）的反应，在后代的相关抗原。这种抑制不是由施用的抗原、怀孕母亲产生的抗体或哺乳转移引起的，而是依赖于完整的母体T细胞的存在。这是主要组织相容性复合体（MHC）限制性方式的耐受性，这种耐受性至少持续了六分之一的海洋生物。传统上，胎盘充当天然屏障，不允许细胞通过。然而，结果强烈表明，母体T细胞通过胎盘，随后诱导耐受。在本研究中，我们试图通过使用分子技术证实母体细胞在胎儿循环中的存在。我们发现，一个高度多态性的微卫星序列内的H-2复杂的II类Eb基因是有用的各种H-2等位基因的分子检测。利用DNA多态性分析技术对小鼠脾脏中的母体H-2等位基因进行了追踪，并根据我们的基本方法，在组织了充分的知情同意后，尝试证明人类系统中的母体细胞运输。对母亲及其11岁和13岁的子女进行HLA位点A、B、C、DR、DQ的检测。但目前还没有获得完整的、显示母细胞HLA位点的母细胞在其子女中的转移。应增加病例，选择SLE等自身免疫综合征患者。