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Molecular biological analysis of eosinophilic sinusitis and otitis media

嗜酸性鼻窦炎和中耳炎的分子生物学分析

基本信息

批准号：
14571625
负责人：
MASUYAMA Keisuke
金额：
$ 2.18万
依托单位：
University of Yamanashi (2003-2004)Kumamoto University (2002)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571625/
关键词：
IL-5 Eotaxin Eosinophil sinusitis otitis media leukotriene ロイコトリエン拮抗剤

项目摘要

To investigate the pathophysiology of eosinophilic sinusitis and otitis media in the upper airways, we have measured cytokine or chemokine mRNAs relevant to eosinophil infiltration in the mucosa. RNA was extracted from the nasal polyps of the subjects suffered from sinusitis. Initial amounts of mRNAs of IL-5, IL-8, GM-CSF, and Eotaxin were calculated using BAS2000 and they were comnpared between recurrent and non-recurrrent groups after surgery. An average of eosinophil infiltration rate in the tissue was statistically increased in the recurrent group compared to non-recurrent one. The expression rates of each cytokine (%) were IL-5(100%), IL-8(88%), Eotaxin(53%), and GM-CSF(24%), respectively and there were no differences in the expression rates between the groups. Eosinophil infiltration rate and initial amout of IL-5 expression showed significant correlation in both groups. However, no correlation was found between Eotaxin or GM-CSF expression and eosinophil infiltration. In recurre … More nt polyps, eosinophil infiltration rate and initial amout of Eotaxin showed significant correlation. Further, there was a trend in correlation between IL-5 and Eotaxin mRNA expressions in the group. These results suggest that the concomitant expressions of IL-5 and Eotaxin mRNAs may induce an increased number of eosinophil infiltration in the upper airways leading to the recurrence of nasal polyps.Next, we have investigated the effect of luekotriene receptor antagonist on eosinophilic sinusitis. An leukotriene receptor antagonist, Pranlukast, was administered for two months to 34 patients who did not respond routine medication. Nasal symptoms and signs were compared before and after medication. Significant reductions in scores were found in rhinorrea, postnasal drip, and easy to blow nose. Amount and character of nasal discharge, and swelling of nasal mucosa also showed significant improvement. These results indicate that leukotrienes produced from eosinophils may be relevant to pathophysiology of eosinophilic sinusitis. Less

为了研究嗜酸性鼻窦炎和上呼吸道中耳炎的病理生理学，我们测定了与粘膜中嗜酸性粒细胞浸润相关的细胞因子或趋化因子mRNA。从鼻窦炎患者的鼻息肉中提取RNA。使用BAS 2000计算IL-5、IL-8、GM-CSF和Eotaxin的初始mRNA量，并在手术后复发组和非复发组之间进行比较。与非复发组相比，复发组组织中的平均嗜酸性粒细胞浸润率在统计学上增加。每种细胞因子的表达率（%）分别为IL-5（100%）、IL-8（88%）、Eotaxin（53%）和GM-CSF（24%），组间表达率无差异。两组患者的嗜酸性粒细胞浸润率与IL-5的初始表达量呈显著相关。然而，Eotaxin或GM-CSF表达与嗜酸性粒细胞浸润之间没有相关性。在复发 ...更多信息结果显示：息肉的大小、嗜酸性粒细胞浸润率与Eotaxin的初始浓度呈显著相关。此外，在该组中IL-5和Eotaxin mRNA表达之间存在相关性的趋势。这些结果表明，IL-5和Eotaxin mRNAs的共同表达可能会诱导嗜酸性粒细胞浸润的上气道导致鼻polyps.Next复发的数量增加，我们已经调查的影响，Eosinophilic鼻窦炎Eosinophilic三烯受体拮抗剂。一种白三烯受体拮抗剂，Pranlukast，给34例常规药物治疗无效的患者服用两个月。比较用药前后鼻部症状和体征的变化。在恶心、后鼻滴涕和容易擤鼻涕方面，得分显著降低。鼻分泌物的量、性质及鼻粘膜肿胀也有明显改善。这些结果表明，嗜酸性粒细胞产生的白三烯可能与嗜酸性鼻窦炎的病理生理。少