Physiological study about skin microcirculation on diabetes mellitus

皮肤微循环与糖尿病的生理学研究

• 批准号: 14571714
• 负责人: HASHIMOTO Ichiro
• 金额: $ 2.18万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571714/
• 关键词: Skin Microcirculation; Flap Necrosis; diabetes mellitus; Transcutaneous PO2; Transcutaneous PCO2

The aim of this study is observation of character and failure of skin microcirculation in diabetes mellitus. Diabetes mellitus model of rabbits and normal rabbits as a control group were used. The following studies were performed.1.Observation of skin microcirculation in the rabbit ear chamber in the diabetes mellitus rabbits and control rabbits.2.Skin flap observations ; random pattern skin flap made on the back of rabbits and island flap made on the abdomen.(1)Transcutaneous PO2 and PCO2 monitoring(2)Dopplar flowmeter(3)Skin necrosisIn the study of the random pattern skin flap, the difference of surviving area between the diabetes mellitus model and the control group was not significant. The reasons of this result are thought to be the blood glucose level, the duration of diabetes mellitus and the flap size. Serotonin (5-hydroxytryptamine) receptor antagonist showed inhibition effects to thrombus formation in arterioles and venules of the rabbit ear chamber by suppression of platelet aggregation. This drug inhibited the distal necrosis of the random pattern flap made on the back of rabbits. Histological findings of the flap revealed patent vessels in the deep dermis of the treated flap and occlusion of vessels in the deep dermis of the control group. These findings showed that flap survival effect of this agent is due to the suppression of the platelet aggregation in vessels of the deep dermis. The next study was histological analysis about skin flap survival effect by antagonist of leukocyte elastase. This agent showed the elongation effect of the skin flap damaged by ischemia reperfusion injury. Histological findings revealed that the infiltration of leukocyte into the skin in the control group was more than the infiltration in the treated goup.

本研究旨在观察糖尿病患者皮肤微循环的特点及障碍。采用家兔糖尿病模型，正常家兔作为对照组。本实验进行了以下几方面的研究：1.糖尿病兔耳腔皮肤微循环的观察; 2.皮瓣的观察：在兔背部制作任意型皮瓣，在腹部制作岛状皮瓣。（1）经皮氧分压（PO_2）、二氧化碳分压（PCO_2）监测（2）多普勒血流仪（Doppler flow）检测（3）皮肤坏死在随机型皮瓣的研究中，糖尿病模型组皮瓣成活面积与对照组比较差异无显著性。其原因可能与血糖水平、糖尿病病程及皮瓣大小有关。5-羟色胺（5-HT）受体拮抗剂通过抑制血小板聚集，抑制兔耳腔微动脉和微静脉血栓形成。本品对家兔背部随意型皮瓣远端坏死有明显的抑制作用。皮瓣的组织学检查结果显示，治疗组皮瓣真皮深层血管通畅，对照组真皮深层血管闭塞。这些结果表明，这种药物的皮瓣成活效果是由于抑制血小板聚集在血管的真皮深部。其次，对白细胞弹性蛋白酶拮抗剂对皮瓣成活的影响进行组织学分析。该制剂对缺血再灌注损伤的皮瓣有延长作用。组织学检查显示，对照组皮肤白细胞浸润多于治疗组。

项目成果

The Features of Thrombus in a Microvessel Injury Model and the Antithrombotic Efficacy of Heparin, Urokinase, and Prostaglandin E1

• DOI: 10.1097/01.prs.0000060796.93369.27
• 发表时间: 2003-06
• 期刊: Plastic and Reconstructive Surgery
• 影响因子: 3.6
• 作者: I. Hashimoto;H. Nakanishi;Y. Shono;Masahiro Yamano;M. Toda

Angiostatic effects of corticosteroid on wound healing of the rabbit ear

皮质类固醇对兔耳伤口愈合的血管抑制作用

• 发表时间: 2002
• 期刊: The Journal of Medical Investigation 49-1,2
• 作者: Takagi K;Takagi K;Kazuya Matsumoto;Takagi K.;Matsumoto K;高木健次郎;Kazuya Matsumoto;高木健次郎;Ichiro Hashimoto;高木健次郎;高木健次郎;Ichiro Hashimoto;橋本一郎;高木健次郎;Hashimoto I;Takagi K;Hashimoto I;Ichiro Hashimoto;Takagi K.;Ichiro Hashimoto;高木健次郎;Hashimoto I
• 通讯作者: Hashimoto I

Occlusal difficulties after simultaneous mandibular and maxillary distraction in an adult case hemifacial microsomia.

成人半面部短小症同时下颌和上颌牵引后出现咬合困难。

• 发表时间: 2004
• 期刊: J Craniofac Surg 15-3
• 作者: Takagi K;Takagi K;Kazuya Matsumoto;Takagi K.;Matsumoto K
• 通讯作者: Matsumoto K

透析患者の手術2003 I)総論 2)各種病態における創傷の治癒

透析患者的手术 2003 I) 概述 2) 各种病理情况下的伤口愈合

• 发表时间: 2003
• 期刊: 臨床透析 19・7
• 作者: Takagi K;Takagi K;Kazuya Matsumoto;Takagi K.;Matsumoto K;高木健次郎;Kazuya Matsumoto;高木健次郎;Ichiro Hashimoto;高木健次郎;高木健次郎;Ichiro Hashimoto;橋本一郎
• 通讯作者: 橋本一郎

Ichiro Hashimoto: "The Feature of Thrombus in a Microvessel Injury Model and the Antithrombotic Efficacy of Heparin, Urokinase, and Prostaglandin E1"Plastic and Reconstructive Surgery. 111・7. 2307-2314 (2003)

桥本一郎：“微血管损伤模型中的血栓特征以及肝素、尿激酶和前列腺素 E1 的抗血栓作用”整形外科 111・7（2003）。