Involvement of reactive oxygen species in flap necrosis and development of its prevention by antioxidants.

活性氧参与皮瓣坏死以及抗氧化剂预防其发展。

• 批准号: 02670542
• 负责人: SUZUKI Shigehiko
• 金额: $ 1.47万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670542/
• 关键词: SOD; Reactive oxygen species; Skin flap; Antioxidant; Lipid peroxide; Fat flap; 過酸化脂貿; 虚血再潅流障害

1)In attempts to substantiate the possible participation of reactive oxygen species, and significance of the xanthine oxidase system in both post-ischemic reperfusion necrosis of the island flap and distal necrosis of the pedicle flap, and to develop new pharmacological measures for salvaging flap necrosis, a series of experiments were made using an island flap model and a random-pattern flap model in rats. The results demonstrated a possible involvement of ROS in both post-ischemic necrosis of island flaps and distal necrosis of random flaps. Xanthine oxidase was significant in producing ROS only in the former.2)In order to examine the effect of topical use of SOD ointment which contains 1% SOD on the enhancement of flap survival, three experiments were made. Its application to the under surface of the flap or occlusive dressing was effective.3)Involvement of lipid peroxidation in flap necrosis and effect of topical application of herbal antioxidant in salvagng flap necrosis were investigated. Among the herbal antioxidants, ellagic acid exerted the strongest effect in suppressing lipid peroxide levels and increasing flap survival.4)Ischemic-reperfusion injury of island fat flap of rats was also investigated.

（1）为了证实活性氧的可能参与，以及黄嘌呤氧化酶系统在岛状皮瓣缺血再灌注后坏死和带蒂皮瓣远端坏死中的意义，并发展新的挽救皮瓣坏死的药理学措施，采用大鼠岛状皮瓣模型和随机皮瓣模型进行了一系列实验。结果表明ROS可能参与了岛状皮瓣缺血后坏死和任意皮瓣远端坏死。黄嘌呤氧化酶仅在前者中产生活性氧。2）为了检测局部使用含1%SOD的SOD软膏对促进皮瓣成活的作用，进行了三个实验。3）探讨了脂质过氧化反应在皮瓣坏死中的作用及局部应用中药抗氧化剂对皮瓣坏死的挽救作用。在抗氧化剂中，鞣花酸在抑制脂质过氧化物水平和提高皮瓣存活率方面的作用最强。4）研究了大鼠岛状脂肪瓣的缺血再灌注损伤。

项目成果

Suzuki, S., Matsushita, Y., Isshiki, N., Hamanaka, H. and Miyachi,Y.: "Salvage of distal flap necrosis by topical superoxide dismutase, Ann." Plast. Surg.27. 253-257 (1991)

Suzuki, S.、Matsushita, Y.、Isshiki, N.、Hamanaka, H. 和 Miyachi,Y.：“通过局部超氧化物歧化酶挽救远端皮瓣坏死，Ann。”

大塚 守正,鈴木 茂彦,一色 信彦: "レーザードップラー組織血流計,熱勾配式組織血流計を用いた皮弁血流測定及び臨床応用について." 日本形成外科学会誌.

Morimasa Otsuka、Shigehiko Suzuki、Nobuhiko Isshiki：“使用激光多普勒组织血流计和热梯度组织血流计测量皮瓣血流及其临床应用。”

Suzuki, S., Yoshioka, N., Isshiki, N., Hamanaka, H. and Miyachi, Y.: "Involvement of reactive oxygen species in postischemic flap necrosis and its prevention by antioxidants." Br. J. Plast. Surg.44. 130-134 (1991)

Suzuki, S.、Yoshioka, N.、Isshiki, N.、Hamanaka, H. 和 Miyachi, Y.：“活性氧在缺血后皮瓣坏死中的参与及其抗氧化剂的预防。”

鈴木 茂彦: "皮弁壊死の予防と対策:皮弁移植法,最近の進歩. 鳥居 修平編" 克誠堂出版,

铃木茂彦：《皮瓣坏死的预防与对策：皮瓣移植方法的最新进展》，鸟井周平主编《Kuseido Publishing》，

Suzuki,S.: "Involvement of reactive oxygen species in postischemic flap necrosis and its prevention" Procceding of the 7th International Conference of Plastic Surgery.

Suzuki,S.：“活性氧参与缺血后皮瓣坏死及其预防”第七届国际整形外科会议记录。