Cyclin G2: Expression and functional analysis of new cell cycle regulator in human oral cancer

Cyclin G2：人类口腔癌中新型细胞周期调节因子的表达和功能分析

• 批准号: 14571750
• 负责人: KOHNO Yohko
• 金额: $ 1.6万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571750/
• 关键词: Cell cycle; Cyclin G2; Cyclin G1; Control factor

We have shown that human cyclin G2 is down-regulated in laser capture microdissection using microarray. Western analysis showed detectable cyclin G2 protein in normal but not in malignant oral keratinocyte cell lines. Immunohistochemistry analysis done on oral mucosa, dysplastic oral epithelia and oral cancer epithelia. Cyclin G2 expressed in 100% of normal oral mucosa, 69.1% of dysplastic oral epithelia in the nuclei and 11.1% of oral cancer epithelia in the nuclei. In vitro functional study by ectopic restoration of Cyclin G2 expression in the human malignant squamous cell carcinoma line resulted in a inhibition of cellular proliferation and colony formation with increased population of G1 phase and decreased in S phase. Stable down-regulation of Cyclin G2 by short interference RNA-based gene silencing in immortalized normal oral keratinocytes resulted in enhanced cell growth with increase in S and prominently in G2 phase. Cyclin G2, has been implicated as a negative regulator in cell cycle progression.

我们已经表明，人类细胞周期蛋白G2下调激光捕获微切割使用微阵列。Western分析表明，可检测到细胞周期蛋白G2蛋白在正常的，但不是在恶性口腔角质形成细胞系。对口腔粘膜、口腔上皮异型增生和口腔癌上皮进行免疫组化分析。Cyclin G2在正常口腔黏膜中表达率为100%，在口腔不典型增生上皮中表达率为69.1%，在口腔癌上皮中表达率为11.1%。在体外功能研究中，通过异位恢复Cyclin G2在人恶性鳞状细胞癌细胞系中的表达，导致细胞增殖和集落形成的抑制，G1期群体增加，S期群体减少。在永生化的正常口腔角质形成细胞中，通过基于短干扰RNA的基因沉默稳定下调细胞周期蛋白G2，导致细胞生长增强，S期增加，G2期显著增加。细胞周期蛋白G2是细胞周期进程中的负调控因子。

项目成果

Apoptosis, proliferation and p12doc-1 profiles in normal, dysplastic and malignant squamous epithelium of the Syrian hamster cheek pouch model

• DOI: 10.1016/s1368-8375(01)00055-0
• 发表时间: 2002-04-01
• 期刊: ORAL ONCOLOGY
• 影响因子: 4.8
• 作者: Kohno, Y;Patel, V;Todd, R
• 通讯作者: Todd, R

Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis

• DOI: 10.1038/sj.onc.1204685
• 发表时间: 2001-09-27
• 期刊: ONCOGENE
• 影响因子: 8
• 作者: Alevizos, I;Mahadevappa, M;Wong, DTW
• 通讯作者: Wong, DTW

Cyclin G2 Dysregulation in human oral cancer

• DOI: 10.1158/0008-5472.can-04-1926
• 发表时间: 2004-12-15
• 期刊: CANCER RESEARCH
• 影响因子: 11.2
• 作者: Kim, Y;Shintani, S;Wong, DT
• 通讯作者: Wong, DT