The effects of the role of cytokine stimulated capsular polysaccharide Actinobacillus actinomycetemcomitans in human gingival fibroblast and monocyte.

我们之前报道了来自放线菌Y4的荚膜多糖（CP）在小鼠器官培养系统中诱导骨吸收和小鼠骨髓培养中破骨细胞的形成。然而，放线菌comitans Y4 CP对人牙龈成纤维细胞（HGF）的影响尚不清楚。本研究旨在验证A. actinomycetemcomitans Y4 CP改变HGF产生炎症细胞因子，如白细胞介素-6 （IL-6）和IL-8的假设。不同浓度的Y4 CP培养HGF 24 h后，IL-6和IL-8的产生呈浓度依赖性降低。Y4 CP （100 μ g/ml）抑制IL-6的释放量从9.09 +/-0.08 ng/ml降至0.34 +/-0.21 ng/ml (P<0.01)， IL-8的生成量从3.76 +/-0.03 ng/ml降至0.09 +/-0.01 ng/ml （P<0.01）。Y4 CP也抑制了由Y4脂多糖（LPS）刺激的HGF释放的70-80%的IL-6和IL-8。有趣的是,从。放线菌Y4 CP完全抑制放线菌Y4 CP对HGF生成IL-6和IL-8的影响。这些结果表明，Y4 CP抑制HGF中IL-6和IL-8的释放，表明放线菌Y4调节牙周炎的炎症反应。值得注意的是，这种抑制作用被特异性抗a逆转。放线菌（actinomycetemcomitans） Y4 CP提示生物体与体液宿主反应之间的重要关系。

We previously reported that a capsular polysaceharide (CP) from Actinobacillus actinomycetemcomitans Y4 induces bone resorption in a mouse organ culture system and osteoclast formation in mouse bone marrow cultures. However, the effects of A. actinomycetemcomitans Y4 CP on human gingival fibroblasts (HGF) are still unclear. The present study was undertaken to test the hypothesis that A. actinomycetemcomitans Y4 CP alters the production of inflammatory cytokines, such as interleukin-6 (IL-6) and IL-8 by HGF. When HGF were cultured with various concentrations of Y4 CP for 24 h, IL-6 and IL-8 production decreased in a concentration-dependent manner. Y4 CP (100 microg/ml) suppressed the release of IL-6 from 9.09 +/-0.08 ng/ml to 0.34 +/-0.21 ng/ml (P<0.01) and IL-8 production decreased from 3.76 +/-0.03 ng/ml to 0.09 +/-0.01 ng/ml (P<0.01). Y4 CP suppressed 70-80% of the release of IL-6 and IL-8 from HGF stimulated with Y4 lipopolysaccharide (LPS), too. Interestingly, anti-A. actinomycetemcomitans Y4 CP completely inhibited the effect of A. actinomycetemcomitans Y4 CP on IL-6 and IL-8 production from HGF. These results indicate that Y4 CP inhibits the release of IL-6 and IL-8 from HGF, suggesting that A. actinomycetemcomitans Y4 modulates the inflammatory response in periodontitis. Remarkably, this inhibitory effect was reversed by specific anti-A. actinomycetemcomitans Y4 CP suggesting an important relationship between the organism and the humoral host response.