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The roll of soluble interleukin-1 receptor type 2 for periodontal tissue

牙周组织可溶性白细胞介素1受体2型卷

基本信息

批准号：
16592082
负责人：
ISHIHARA Yuichi
金额：
$ 2.3万
依托单位：
Aichi-Gakuin university
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Head investigator and investigators have provided the results from this grant projects.(1)One hundred and eighty one gingival crevicular fluids samples were obtained from 52 periodontitis patients and interleukin-1 (IL-1)β and soluble IL-1 receptor type 2 (sIL-1Rt2) were measured by using ELISA assay kits. The concentration of IL-1β and sIL-1t2 were positive correlated with the periodontal disease severity. On the other hand, GCF IL-1b and sIL-1t2 concentrations from chronic periodontitis (CP) patients and aggressive periodontitis (AgP) patients were also examined. IL-1β was no different between CP (82.2±12.3ng/ml)and AgP(68.3±9.4ng/ml), however, AgP sIL-1Rt2(3.0±0.6ng/ml) was significantly decreased compared with CP sIL-1Rt2(1.1±0.3ng/ml). Therefore, these results suggest that AgP disease character of rapid periodontal tissue breakdown may relate to the decreased sIL-1Rt2 production. (2)A total of 102 Japanese patients and 88 periodontally healthy volunteers were recruited at the Depa … More rtment of Periodontology in Aichi-gakuin University from 2002 to 2004. Polymorphisms were identified by direct sequence method. After the single nucleotide polymorphisms (SNPs) identification, allelo- and haplotype analysis were performed. A 14.5-kb genomic region containing all exons and relevant exon-intron boundaries of IL1R1 and IL1R2 were sequenced in 24 AgPs and 24 controls. Within genomic regions containing all exons and relevant exon-intron boundaries in IL1R1 and IL1R2,38 SNPs were identified in 48 subjects. Five were located within coding elements, 2 in the 5' flanking region,22 within introns, and 9 in the 3' untranslated region. Frequency of 3 SNPs and 3 haplotypes in IL1R2 showed significant differences between patients with AgP and controls (p<0.05). (3)Gingival fibroblasts were isolated from chronic periodontitis patients. And cells were stimulated with Actinobacillus actinomycetemcomitans lipopolysaccharide, which is strongly associated with AgP. IL-1β and IL-1Rt2 mRNA gene expression were measured. However, protein production was only measured for IL-1β and not for sIL-1Rt2 in culture supernatant. Project is now undergoing for different experimental design using immunocompetent cell such as neutrophil or mononuclear cells. Less

首席调查员和调查员提供了这项赠款项目的结果。（1）收集52例牙周炎患者181份龈沟液，采用酶联免疫吸附法（ELISA）检测IL-1 β和可溶性IL-1受体2（sIL-1 Rt 2）。IL-1β、sIL-1 t2水平与牙周病严重程度呈正相关。同时检测了慢性牙周炎（CP）和侵袭性牙周炎（AgP）患者龈沟液中IL-1b和sIL-1 t2的含量。IL-1β在CP组（82.2±12.3ng/ml）和AgP组（68.3±9.4ng/ml）无显著性差异，而sIL-1 Rt 2在AgP组（3.0±0.6ng/ml）明显低于CP组（1.1±0.3ng/ml）。因此，这些结果表明，AgP疾病的特点，快速牙周组织破坏可能与减少sIL-1 Rt 2的生产。(2)A在Depa共招募了102名日本患者和88名牙周健康志愿者 ...更多信息 2002年至2004年在爱知学院大学进行牙周病学研究。直接测序法鉴定多态性。单核苷酸多态性（SNPs）鉴定后，进行等位基因和单倍型分析。在24个AgP和24个对照中，对包含IL 1 R1和IL 1 R2的所有外显子和相关外显子-内含子边界的14.5-kb基因组区域进行测序。在包含IL 1 R1和IL 1 R2的所有外显子和相关外显子-内含子边界的基因组区域内，在48名受试者中鉴定出38个SNP。5个位于编码元件内，2个位于5'侧翼区，22个位于内含子内，9个位于3'非翻译区。IL 1 R2基因3个单倍型和3个SNP的频率在AgP组和对照组之间有显著性差异（p<0.05）。（3）分离慢性牙周炎患者牙龈成纤维细胞。用与AgP密切相关的伴放线放线杆菌脂多糖刺激细胞。检测IL-1β和IL-1 Rt 2 mRNA表达。然而，在培养上清液中仅测量IL-1β的蛋白质产生，而不测量sIL-1 Rt 2。项目目前正在进行不同的实验设计，使用免疫活性细胞，如中性粒细胞或单核细胞。少