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The specificity of cytokine production from inflammatory gingiva

炎症牙龈产生细胞因子的特异性

基本信息

批准号：
12672044
负责人：
ISHIHARA Yuichi
金额：
$ 2.05万
依托单位：
Aichi Gakuin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

Periodontitis is a general term for disease categories, including juvenile periodontitis (JP), rapidly progressive periodontitis (RPP), and adult periodontitis (AP), which may or may not share a common etiology and pathogenesis. These disease categories are characterized by difference in progression of tissue destruction and differences in age group susceptibility, but not to our knowledge, by differences in cytokine responses of inflammatory cells. Therefore, the purpose of this study is to investigate the difference of the levels of gingival crevicular Interleukin-1 (IL-1) molecules and the clinical status ot patients with different categories of periodontitis. IL-1 alfa, IL-1 beta, IL-1 receptor antagonist (ra) and the total IL-1/IL-1 ra ratio (IL-1 activity index ; IL-1AI) were measured in 353 gingival crevicular fluid (GCF) samples from 45 non-inflamed gingiva sites in 9 healthy subjects and 215 diseased sites in 50 patients with AP and 93 diseased sites in 18 patients with RPP. IL-1 alfa, IL-1 beta and IL-1 ra were measured by specific non-crossreactive enzyme linked immunosorbent assay (ELISA). The probing depth, gingival index and alveolar bone loss of each site was recorded at the time of GCF sampling. The total amount of IL-1 beta in RPP GCF was higher than AP GCF, however, the total amount of IL-1 alfa was approximately same levels in both AP and RPP GCF. The RPP IL-1AI was significantly higher than AP IL-1AI (p<0.05). The levels of IL-1 molecules in culture supernatant fluids of peripheral blood lymphocyte (PBL), which were cocultured with bacterial lipopolysaccharide, were also compared between AP and RPP. RPP PBL induced more IL-1 beta than AP PBL also. These data suggest that RPP cytokine responses of inflammatory cells are higher than AP and this phenomenon may relate to rapid and sever penodontal tissue destruction in RPP.

牙周炎是疾病类别的总称，包括青少年牙周炎（JP）、快速进展性牙周炎（RPP）和成人牙周炎（AP），其可能具有或可能不具有共同的病因和发病机制。这些疾病类别的特征在于组织破坏进展的差异和年龄组易感性的差异，但据我们所知，不是炎性细胞的细胞因子反应的差异。因此，本研究旨在探讨不同类型牙周炎患者龈沟液中白细胞介素-1（IL-1）分子水平的差异及其与牙周炎临床症状的关系。检测9例健康人45个非炎症牙龈、50例AP患者215个病变牙龈和18例RPP患者93个病变牙龈的353份龈沟液（GCF）中IL-1 α、IL-1 β、IL-1受体拮抗剂（ra）和总IL-1/IL-1 ra比值（IL-1活性指数; IL-1AI）。采用特异性非交叉反应酶联免疫吸附试验（ELISA）检测IL-1 α、IL-1 β和IL-1 ra。在GCF采样时记录每个部位的探诊深度、牙龈指数和牙槽骨丢失情况。RPP GCF中IL-1 β的总量高于AP GCF，而AP和RPP GCF中IL-1 α的总量大致相同。RPP组IL-1AI显著高于AP组（p<0.05）。比较AP和RPP患者外周血淋巴细胞（PBL）与细菌脂多糖共培养上清液中IL-1分子水平的变化。RPP PBL比AP PBL诱导更多的IL-1 β。提示RPP时炎性细胞的细胞因子反应高于AP，这种现象可能与RPP时阴茎组织破坏迅速而严重有关。