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Identification and mechanical analysis of the factor from human periodontal ligaments with bone remodeling by tooth movement

人牙周膜牙齿移动骨重建的影响因素识别及力学分析

基本信息

批准号：
14571952
负责人：
OBA Yasuo
金额：
$ 2.24万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

PDL (periodontal ligament) cells are recognized as regulation of mechanical stress that should be able to exchange the mechanical stress to the biological signaling and thought to express a set of unique genes under the regulation of their own mechanism, but details of the gene expression pattern in response to mechanical stress is still unclear. In this study, to identify mechanical stress-related genes in human PDL cells, the differential expression level of genes in PDL cells stimulated by mechanical stress was examined using cDNA microarray analysis.1.Application of compressive forceIn order to determine the effect of static compressive force on the PDL cells, cells were embedded and cultured in a three-dimensional collagen gel system to minic in vivo conditions. Compressive forces (mechanical stress) were applied by the use of a plastic cylinder placed over the gels. The gels without the weight loading served as control.2.Identification of genes in response to mechanical stress by the cDNA microarray analysisIn the microarray analysis, 108 independent genes related to mechanical stress were identified. Among them, 85 genes were up-regulated by mechanical stress, on the other hand, 23 genes were down-regulated.In this study, first we developed the three-dimensional collagen gel cell culture system that resembles in vivo condition to determine the gene profile regulated by a static compressive force in PDL cells. Secondary, we attempted to dentify the gene profiles in response to mechanical stress in PDL cells using cDNA microarray technology. Novel and interesting genes related to mechanical stress were expressed, and also found the genes in which the function is unclear. Further studies on their physiological roles may contribute to clarify the mechanism of periodontal tissue during tooth movement.

牙周膜细胞被认为是机械应力的调节细胞，应该能够将机械应力转换为生物信号，并被认为在其自身机制的调控下表达一组独特的基因，但其响应机械应力的基因表达模式的细节尚不清楚。本研究采用cDNA微阵列技术检测了机械应力对人牙周膜细胞基因表达水平的影响，以筛选出与机械应力相关的基因。1.压力的应用为了确定静态压力对牙周膜细胞的影响，将细胞包埋并培养在三维胶原凝胶系统中以模拟体内条件。通过使用放置在凝胶上的塑料圆筒施加压缩力（机械应力）。2.利用基因芯片技术筛选机械应力相关基因在基因芯片中，共筛选出108个与机械应力相关的独立基因。在本研究中，我们首先建立了一个类似于体内条件的三维胶原凝胶细胞培养系统，以确定静态压力对PDL细胞基因表达的影响。其次，我们尝试用cDNA微阵列技术鉴定PDL细胞对机械应力反应的基因谱。表达了与机械应力相关的新的、有趣的基因，也发现了功能尚不清楚的基因。进一步研究它们的生理作用有助于阐明牙周组织在牙齿移动过程中的作用机制。