The three-demensional architecture of the microcirculation (blood and lymphatic vessel) in experimental periodontitis

牙周组织特别是牙周韧带和牙槽骨的微循环有血管和淋巴管两种。由于以下原因，观察整个牙周组织的微循环一直是非常困难的。2.复杂的位置为硬组织的存在；2 .在载玻片上进行三维和/或动态观察的难度；血液和淋巴管分离的困难。但是，现在，各种特异性淋巴标记物的鉴定极大地促进了微循环的研究。本研究显示了实验性牙周组织中血液和淋巴管的结构。免疫组织化学发现，CD34染色的血管未被CD9和D2-40染色，被血管内红细胞和平滑肌细胞层识别。相反，CD9和D2-40染色了未被CD34染色的淋巴管，通过具有波浪形轮廓的扁平内皮和极弱的细胞质，缺乏血管内红细胞以及平滑肌细胞层来识别。实验组血、淋巴管数量随时间增加而增加。

There is two kinds of the micro-circulation, both vascular and lymphatic vessels in the periodontal tissue, especially periodontal ligament and alveolar bone. It has been very difficult, to observe the whole micro-circulation in the periodontal tissue because of the following reasons, 1.the complex location for the existence of the hard tissue, 2.the difficulty of the 3-D and/or dynamic observation on the glass slide, and 3.the difficulty of separation of blood and lymphatic vessel. But, now, the identification of various specific lymphatic markers has greatly facilitated studies of the micro-circulation. The present study showed the architecture of the blood and lymphatic vessels in the periodontal tissue in the experimental periodontal tissue. In immunohistochemical finding, CD34 stained blood vessels that did not stain with CD9 and D2-40, recognized by intravascular erythrocytes and a smooth muscle cell layer.Conversely, CD9 and D2-40 stained lymphatic vessels that did not stain with CD34, recognized by flat-lined endothelium with a wavy outline and extremely attenuated cytoplasm, the lack of intravascular erythrocytes, as well as a smooth muscle cell layer. The number of blood and lymphatic vessels increased with the time in experimental group.