The three-demensional architecture of the microcirculation (blood and lymphatic vessel) in experimental periodontitis

牙周组织中存在血管和淋巴管两种微循环，尤其是牙周膜和牙槽骨。由于硬组织存在的部位复杂，玻璃片上的三维和/或动态观察困难，以及血管和淋巴管分离困难等原因，牙周组织的微循环观察一直是非常困难的。但是，现在，各种特定的淋巴标志物的识别极大地促进了微循环的研究。本研究显示了实验牙周组织中血管和淋巴管的构筑。免疫组织化学显示CD34染色不表达CD9和D2-40的血管，可被血管内红细胞和一层平滑肌层识别；CD9和D2-40表达不表达CD34的淋巴管，被扁平的内皮识别，轮廓呈波浪形，细胞质极稀薄，血管内缺乏红细胞，并有一层平滑肌层。实验组血管和淋巴管数量随时间延长而增加。

There is two kinds of the micro-circulation, both vascular and lymphatic vessels in the periodontal tissue, especially periodontal ligament and alveolar bone. It has been very difficult, to observe the whole micro-circulation in the periodontal tissue because of the following reasons, 1.the complex location for the existence of the hard tissue, 2.the difficulty of the 3-D and/or dynamic observation on the glass slide, and 3.the difficulty of separation of blood and lymphatic vessel. But, now, the identification of various specific lymphatic markers has greatly facilitated studies of the micro-circulation. The present study showed the architecture of the blood and lymphatic vessels in the periodontal tissue in the experimental periodontal tissue. In immunohistochemical finding, CD34 stained blood vessels that did not stain with CD9 and D2-40, recognized by intravascular erythrocytes and a smooth muscle cell layer.Conversely, CD9 and D2-40 stained lymphatic vessels that did not stain with CD34, recognized by flat-lined endothelium with a wavy outline and extremely attenuated cytoplasm, the lack of intravascular erythrocytes, as well as a smooth muscle cell layer. The number of blood and lymphatic vessels increased with the time in experimental group.