The three-demensional architecture of the microcirculation (blood and lymphatic vessel) in experimental periodontitis

牙周组织中存在着血管和淋巴管两种微循环，特别是牙周膜和牙槽骨。由于硬组织存在的位置复杂、载玻片上的三维和/或动态观察困难、血液和淋巴管分离困难等原因，观察牙周组织中的整个微循环是非常困难的。但是，现在，各种特异性淋巴标记物的鉴定极大地促进了微循环的研究。本研究显示了实验性牙周组织中血管和淋巴管的构筑。在免疫组化中，CD 34染色的血管未被CD 9和D2-40染色，由血管内红细胞和平滑肌细胞层识别;相反，CD 9和D2-40染色的淋巴管未被CD 34染色，由具有波浪状轮廓和极度衰减的细胞质的平坦衬里的内皮识别，缺乏血管内红细胞，以及平滑肌细胞层。实验组血管和淋巴管数量随时间延长而增加。

There is two kinds of the micro-circulation, both vascular and lymphatic vessels in the periodontal tissue, especially periodontal ligament and alveolar bone. It has been very difficult, to observe the whole micro-circulation in the periodontal tissue because of the following reasons, 1.the complex location for the existence of the hard tissue, 2.the difficulty of the 3-D and/or dynamic observation on the glass slide, and 3.the difficulty of separation of blood and lymphatic vessel. But, now, the identification of various specific lymphatic markers has greatly facilitated studies of the micro-circulation. The present study showed the architecture of the blood and lymphatic vessels in the periodontal tissue in the experimental periodontal tissue. In immunohistochemical finding, CD34 stained blood vessels that did not stain with CD9 and D2-40, recognized by intravascular erythrocytes and a smooth muscle cell layer.Conversely, CD9 and D2-40 stained lymphatic vessels that did not stain with CD34, recognized by flat-lined endothelium with a wavy outline and extremely attenuated cytoplasm, the lack of intravascular erythrocytes, as well as a smooth muscle cell layer. The number of blood and lymphatic vessels increased with the time in experimental group.