Sequence specific double-strand DNA cleavage by an optical active dinuclear iron complex and application for artificial restriction enzyme

1-1）我的方法是创建金属复合体，该复合体能够完全和选择性地清除双链DNA（如bleomycin，哪种是一种抗生素或抗生素，它导致DNA在5'-GC-3'或5'-GT-3'位点上的单一和双链断裂），我准备了一种光学活性五聚氰胺配体， N，N，n'-tri（2-pyridylmethyl）-2-S-（氨基甲基）吡啶= S-P3吡啶从S-2-氨基吡啶的反应中获得，含有3个相等量的2-氯甲基吡啶，并研究铜（II）和镍（II）复合物的X射线晶体结构，[Cu]（S-P3pipda）]（ClO_4）_2和[Ni]（S-P3 pipda）（OH_2）]（ClO_4）_2复合体， [Cu]（S-P3pipda）]（ClO_4）_2和[Ni]（S-P3pipda）（OH_2）]（ClO_4）_2，were prepared from the reaction of an equimolar amount of Cu（ClO_4）_2·6H_2 O or Ni（SO_4）_2·6H_2 O（由LiClO_4的添加物）配体S-P3管在argon下的甲醇。[Cu]的X射线晶体结构（S-P3 pida）]^<2+>显示在两种第三胺中的三角形和正方形金字塔之间的中间结构，在两种第三胺中，equatrial position被占用。（N2 and N3） and two pyridine nitrogens （N1 and N4） and axial position by a pyridine nitrogen （N5）。渗透离子偶氮变弱： Cu-O是3.55.1 -2）我们合成了1，8-（N-羟基甲基）-二（N'，N'-二酰甲基-R，R-环己酮-1，2-二胺）， 1，并在空气中对1 DNA的清除活动进行了评估。一种新型的Fe复合体1和钠冷凝器系统清除了DNA丰富的A-T区域.（2）Moreover，我合成了两个新的核平台（II）复合体， 1，3-和1，4-（benzenedimethanethiolate-S）di（2，2'，2'-terpyridine）platinum（II） chloride tetrahydrate （2 and 3）， were synthesized in order to investigate the binding of platinum（II） complexes with calf thymus DNA，这些都是由紫外线和CD光谱学进行的。复合体2与DNA紧密相互作用，通过相互作用比较为3。

1-1)In my approach to create metal complexes that are capable of cleaving double-strand DNA simultaneously and sequence-selectively (like bleomycin, which is an antitumor antibiotic which causes single and double strand cleavage of DNA at 5'-GC-3' or 5'-GT-3' sites), I prepared an optical active pentadentate ligand, N,N,N'-tri(2-pyridylmethyl)-2-S-(aminomethyl)piperidine = S-P3pipda obtained from the reaction of S-2-aminopiperidine with 3 equimolar amounts of 2-chloromethylpyridine, and investigated the X-ray crystal structures of copper(II) and nickel(II) complexes of S-P3pipda, [Cu(S-P3pipda)](ClO_4)_2 and [Ni(S-P3pipda)(OH_2)](ClO_4)_2 Complexes, [Cu(S-P3pipda)](ClO_4)_2 and [Ni(S-P3pipda)(OH_2)](ClO_4)_2, were prepared from the reaction of an equimolar amount of Cu(ClO_4)_2・6H_2O or Ni(SO_4)_2・6H_2O (followed by addition of LiClO_4) with the ligand S-P3pipda in methanol under argon.The X-ray crystal structure of [Cu(S-P3pida)]^<2+> shows an intermediate structure between trigonal bipyramid and square pyramid in which equatrial positions are occupied by two tertiary amines (N2 and N3) and two pyridine nitrogens (N1 and N4) and axial position by a pyridine nitrogen (N5). Perchlorate ion coordinates weakly : Cu-O is 3.55 Å.1-2)We synthesized 1,8-(N-anthranylmethyl)-bis(N',N'-dipyridylmethyl-R,R-cyclohexane-1,2-diamine), 1, and cleaving activity of 1 to DNA was evaluated in the presence of sodium ascorbate under air. A novel Fe complex 1 and sodium ascorbate system cleaved A-T rich region of DNA.2)Moreover, I have synthesized two new dinuclear platinum(II) complexes, 1,3-and 1,4-(benzenedimethanethiolate-S)di(2,2',2"-terpyridine)platinum(II) chloride tetrahydrate (2 and 3), were synthesized in order to investigate the binding of platinum(II) complexes with calf thymus DNA, which was examined by UV and CD spectroscopies. Complex 2 interacted strongly with DNA by intercalation compared to 3.