Development of fluorescent probes for metallo-beta-lactamase-producing Gram-negative bacteria

金属β-内酰胺酶几乎能降解几乎所有的β-内酰胺类药物，包括碳青霉烯类。在疾病的初始阶段，确认感染细菌中金属β-内酰胺酶的产生对于有效的化疗是非常重要的。合成了一种基于修饰头孢菌素的新型荧光探针，通过荧光光谱的测量，检测了该荧光探针对金属β-内酰胺酶(IMP-1)的检测能力。随着IMP-1浓度的增加，荧光探针的荧光强度增加。因此，光谱数据支持我们的设计策略的有效性。这种荧光探针将作为先导化合物，用于开发选择性金属β-内酰胺酶感染性细菌的检测试剂。

Metallo-beta-lactamase can hydrolyze nearly almost beta-lactams, including carbapenems. It is very important to confirm the production of metallo-beta-lactamase in infectious bacteria for effective chemotherapy at the initial stages of a disease. A new fluorogenic probe based on modified cephalosporin was synthesized and examined the ability of the fluorogenic probe to detect metallo-beta-lactamase (IMP-1) by measurement of the fluorescence spectra. As the concentration of IMP-1, the fluorescence intensity of the fluorogenic probe increased. Thus, the spectral data supports the validity of our design strategy. This fluorogenic probe will serve as lead compounds for the development of detection agents that are selective metallo-beta-lactamases-producing infectious bacteria.