Analysis of the molecular mechanism of cancer progression

癌症进展的分子机制分析

• 批准号: 14572046
• 负责人: AZUMI Kaoru
• 金额: $ 2.24万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14572046/
• 关键词: breast cancer; progression; differencial display; gene expression; metastasis; colon cancer; CD9; proHB-EGF; EGFR; ラットオリゴDNAチップ; DNAマイクロアレイ; 悪性化; ER-1細胞; ERpP; HBL-100細胞; MDA-MB-453細胞; DNA細胞マイクロアレイ

Tumor progression is the process by which tumor cells acquire more malignant properties, such as invasiveness and metastasis, during tumor development. To elucidate mechanisms of tumor progression, we carried out a differential display analysis to evaluate mRNAs differentially expressed between ER-1 cells which are weakly tumorigenic and non-metastatic, and ERpP cells which are strong tumorigenic and metastatic. The expression levels of five known genes, such as, nm23, thrombospondin 1 (THBS1), GRP78, lysosomal membrane glycoprotein (lamp-2), GATA-3, and unknown gene 18c were strongly increased in ERpP cells. Furthermore, THBS1, lamp-2, GATA-3 and human 18c was increased in malignant human colon cancer cells, as same as in ERpP. Northern blot analysis showed that human 18c strongly expressed in lung tumor, while weakly expressed in breast, ovary, and uterus tumors, respectively. In kidney, stomach and colon tumors, human 18c little expressed. This result indicates that human 18c gene will be useful as a marker gene for lung tumor.

肿瘤进展是肿瘤细胞在肿瘤发展过程中获得更多恶性特性的过程，如侵袭和转移。为了阐明肿瘤进展的机制，我们进行了差异显示分析，以评估弱致瘤性和非转移性ER-1细胞和强致瘤性和转移性ERpP细胞之间的差异表达。在ERpP细胞中，nm23、血栓反应蛋白1(THBS1)、GRP78、溶酶体膜糖蛋白(LAMP-2)、GATA-3和未知基因18c等5个已知基因的表达水平显著升高。此外，THBS1、LAMP-2、GATA-3和人18c在恶性结肠癌细胞中的表达增加，与ERpP相同。Northern印迹分析表明，人18c在肺肿瘤中强表达，在乳腺、卵巢和子宫肿瘤中弱表达。在肾脏、胃和结肠肿瘤中，人18c几乎不表达。这一结果表明人18c基因有可能作为肺癌的标记基因。

项目成果

Kaoru Azumi 他: "Construction of a cDNA microarray derived from the ascidian Ciona a intestinalis"Zoological Science. 20. 1223-1229 (2003)

Kaoru Azumi 等：“源自海鞘的 cDNA 微阵列的构建”《动物学科学》，20. 1223-1229 (2003)。

Kaoru Azumi 他: "A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidian"Marine Environmental Research. (印刷中). (2004)

Kaoru Azumi 等人：“用于分析暴露于三丁基锡的海鞘中的整体基因表达谱的 cDNA 微阵列技术”海洋环境研究（2004 年出版）。

Kazuhito Shida 他: "Hemocytes of Ciona intestinalis express multiple genes involved in innate immune host defense."Biochemical and biophysical research communications. 302. 207-218 (2003)

Kazuhito Shida 等人：“海鞘血细胞表达参与先天免疫宿主防御的多种基因。”生物化学和生物物理研究通讯。 302. 207-218 (2003)

P.Dehal 他: "The draft genome of Ciona intestinalis : Insights into chordate and vertebrate origins"Sience. 298. 2157-2167 (2002)

P. Dehal 等人：“海鞘基因组草图：脊索动物和脊椎动物起源的见解”《科学》298. 2157-2167 (2002)。

Kaoru Azumi 他: "Genomic analysis of immunity in a Urochordate and the emergence of the vertebrate immune system : waiting for Godot"Immunogenetics. 55. 570-581 (2003)

Kaoru Azumi 等：“尾索动物免疫的基因组分析和脊椎动物免疫系统的出现：等待戈多”免疫遗传学 55. 570-581 (2003)