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EFFECTS OF PHYSICAL TRAINING-INDUCED SKELETALMUSCLE TRANSCRIPTIONAL ACTIVAIONS ON GLUCOSE METABOLISM

体能训练引起的骨骼肌转录激活对葡萄糖代谢的影响

基本信息

批准号：
14580046
负责人：
TABATA Izumi
金额：
$ 2.24万
依托单位：
National Institute of Health and Nutrition
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580046/
关键词：
PGC-1 Swimming exercise Running exercise AMPK AICAR Epitrochlearis Soleus ヒラメ筋 PGC-1 )走運動

项目摘要

The purpose of this study was to elucidate the mechanisms underlying low-intensity-exercise-induced peroxisome proliferator-activated receptor γ coactivator-1 α (PGC-1 α) protein expression in rat skeletal muscles. Rats (5-6-week-old) swam without a load and ran on the treadmill at a speed of 13 m/min, respectively, in two 3-h sessions separated by 45 min of rest. PGC-1 α content in epitrochlearis muscle (EPI) was increased by 75 % and 95 %, immediately and 6-h post-swimming, respectively, with no increase in PGC-1 α content in the soleus (SOL). After running, PGC-1 α content in EPI was unchanged, while a 107 % increase in PGC- 1 α content was observed in SOL 6-h post-running. Furthermore, in EPI and SOL as well as other muscles (triceps, plantaris, red and white gastrocnemius), PGC-1 α expression was enhanced concomitant with reduced glycogen post-exercise, suggesting that expression of PGC-1 α occurs in skeletal muscle recruited during exercise.Six-hour after in vitro electrical stimulation, mRNA of PGC-1 α increased in epitrochlearis muscle dissected from sedentary rat. PGC-1 α content in EPI was increased after 18-h in vitro incubation with 0.5 mM 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) and 4 mM caffeine. However, AICAR incubation did not affect PGC-1 α content in the SOL, while PGC-1 α content in the SOL was increased after caffeine incubation. These results suggest that exercise-induced PGC-1 α expression in skeletal muscle may mediated by at least two exercise-induced signaling factors : AMPK activation and Ca2+ elevation. The number of factors involved (both AMPK and Ca2+, or Ca2+ only) in exercise-induced PGC-1 α expression may differ among muscles.

本研究旨在探讨低强度运动诱导大鼠骨骼肌过氧化物酶体增殖物激活受体γ共激活因子1 α（PGC-1 α）蛋白表达的机制。大鼠（5-6周龄）无负荷游泳，并在跑步机上以13米/分钟的速度跑步，分别在两个3小时的会议间隔45分钟的休息。在游泳后即刻和6 h，上滑车肌（EPI）中PGC-1 α含量分别增加了75%和95%，而比目鱼肌（SOL）中PGC-1 α含量没有增加。运行后，EPI中的PGC-1 α含量无变化，而SOL中运行后6 h的PGC- 1 α含量增加了107%。此外，在EPI和SOL以及其他肌肉（三头肌、跖肌、红腓肠肌和白色腓肠肌）中，运动后PGC-1 α的表达增强，同时糖原减少，表明PGC-1 α的表达发生在运动中募集的骨骼肌中。EPI中PGC-1 α含量在体外与0.5 mM 5-氨基咪唑-4-甲酰胺核糖核苷（AICAR）和4 mM咖啡因孵育18 h后增加。AICAR孵育不影响SOL中PGC-1 α的含量，而咖啡因孵育后SOL中PGC-1 α的含量增加。这些结果表明，运动诱导的骨骼肌PGC-1 α表达可能通过AMPK激活和Ca 2+升高这两种运动诱导的信号传导因子介导。运动诱导的PGC-1 α表达中涉及的因素（AMPK和Ca 2+，或仅Ca 2+）的数量在肌肉中可能不同。