A STUDY ON MECHANISMS OF MITOCHONDRIAL BIOGENESIS BY PHYSICAL EXERISE-INDUCED PGC-1 α EXPRESSION

体育锻炼诱导PGC-1α表达的线粒体生物发生机制研究

• 批准号: 16300215
• 负责人: TABATA Izumi
• 金额: $ 9.47万
• 依托单位: National Institute of Health and Nutrition
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16300215/
• 关键词: PGC-1α; Swimming Exercise; High intensity exercise; Low intensity exercise; Epitrochlearis; Soleus; 高強度運動; 低強度運動; epitrochlearis; ヒラメ筋

First, we measured peroxisome proliferator-activated receptor γ coactivator-1 α(PGC-1 α) protein content in the skeletal muscles of male SD rats (age : 5-6 weeks old) after a single session of high-intensity intermittent exercise (HIE) or low-intensity prolonged swimming exercise (LIE). During HIE, the rats swam for fourteen 20-second periods carrying a weight (14% of body weight), and the periods of swimming were separated by a 10-s pause. LIE rats swam with no load for 6 hours in two 3-h sessions, separated by 45 minutes of rest. After HIE, the PGC-1 α protein content in rat epitrochlearis (EPI) muscle had increased by 126, 140, and 126 % at 2h, 6h, and 18h, respectively, compared with that of the control rats. Immediately, 6h, and 18-h after LIE, the PGC-1 α protein content in the muscle was significantly elevated by 84, 95, and 67%, respectively. However, there was no statistically significant difference between the two values.To elucidate dose-response relationship between number … More of exercise bouts during training and training-induced expression of PGC-1α, GLUT-4, and mitochondrial proteins in rat skeletal muscle. In year 2005, Thirty two 5-w old SD rats were randomly assigned to a control group and three exercise training groups. Five days swimming training consisted of three (HIT3), or seven (HIT7), or fourteen (HIT14) bouts of 20-s high-intensity swimming exercise with 10-s pause between the exercise bouts. As a result, the GLUT-4 protein content in rat EPI muscle increased by 75, 83, and 71%, 18-hour after the last session of HIT3, HIT7, and HIT14, respectively, compared with the control rats' muscle. Citrate synthase activity in the muscle was elevated by 23 (HIT3), 23 (HIT7) and 25% (HIT14). There were no significant differences in neither GLUT-4 nor citrate synthase activity among the three training groups. The protein content of PGC-1α was also 61 (HIT3), 43 (HIT7), and 22% (HIT14) higher than the control values.In conclusion, the present investigation suggests that irrespective of the intensity and duration (set) of the exercise training, PGC-1α, GLUT-4, and citrate synthase protein content in rat skeletal muscle increases to a comparable level when stimuli induced by different protocols are saturated. Less

首先，我们测量了雄性SD大鼠（年龄：5-6周龄）在单次高强度间歇运动（HIE）或低强度长时间游泳运动（LIE）后骨骼肌中过氧化物酶体增殖物激活受体γ辅激活物-1α（PGC-1α）蛋白含量。在 HIE 期间，大鼠背负重物（体重的 14%）游泳 14 个 20 秒的周期，游泳周期之间有 10 秒的暂停。 LIE 大鼠在两次 3 小时的训练中空载游泳 6 小时，中间休息 45 分钟。 HIE后，与对照大鼠相比，大鼠滑车上肌（EPI）肌肉中PGC-1α蛋白含量在2小时、6小时和18小时分别增加了126%、140%和126%。 LIE 后立即、6 小时和 18 小时，肌肉中的 PGC-1 α 蛋白含量分别显着升高 84%、95% 和 67%。然而，这两个值之间没有统计学上的显着差异。 旨在阐明训练期间的运动次数与训练诱导的大鼠骨骼肌中 PGC-1α、GLUT-4 和线粒体蛋白表达之间的剂量反应关系。 2005年，将32只5周龄SD大鼠随机分为对照组和3个运动训练组。五天的游泳训练包括三轮（HIT3）、七轮（HIT7）或十四轮（HIT14）20 秒的高强度游泳练习，练习之间有 10 秒的暂停。结果，与对照大鼠肌肉相比，在最后一次HIT3、HIT7和HIT14训练后18小时，大鼠EPI肌肉中的GLUT-4蛋白含量分别增加了75%、83%和71%。肌肉中柠檬酸合酶活性分别升高 23% (HIT3)、23% (HIT7) 和 25% (HIT14)。三个训练组之间的 GLUT-4 和柠檬酸合酶活性均无显着差异。 PGC-1α 的蛋白质含量也比对照值高 61%（HIT3）、43%（HIT7）和 22%（HIT14）。 总之，本研究表明，无论运动训练的强度和持续时间（组数）如何，当不同方案诱导的刺激时，大鼠骨骼肌中 PGC-1α、GLUT-4 和柠檬酸合酶的蛋白质含量增加到可比较的水平。 饱和。较少的