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Pharmacokinetics and residues of oxytetracycline and oxolinic acid in black tiger shrimp (Penaeus monodon) and the effect of cooking on the residues

土霉素和奥索林酸在黑虎虾（斑节对虾）中的药代动力学和残留以及蒸煮对其残留的影响

基本信息

批准号：
14580166
负责人：
UNO Kazuaki
金额：
$ 1.98万
依托单位：
Aichi Konan College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

The pharmacokinetics and residues of oxytetracycline (OTC) and oxolinic acid (OA) were examined in black tiger shrimp (Penaeus monodon, 18-25 g shrimp) after intra-sinus (10 mg/kg) and oral (50 mg/kg) administration. The shrimp were kept in tanks with seawater at a salinity of 5-10 ppt. The water temperature was adjusted to 28-29℃. The hemolymph concentrations of both drugs after intra-sinus dosing were best described by a two-compartment open model. The following equations were obtained to describe the time course of hemolymph levels for two drugs :Oxytetracycline : Ct=35.4・exp(-3.5303・t)+10.6・exp(-0.0429・t)Oxolinic acid : Ct=9.96・exp(-0.8252・t)+3.88・exp(-0.0391・t)The distribution and elimination half-lives were found to be 0.2 and 16.2 h for OTC and 0.84 and 17.7 h for OA. The apparent volume of distribution at a steady state and total body clearance were estimated to be 869 ml/kg and 38.7 ml/kg/h for OTC and 2,061 ml/kg and 90.1 ml/kg/h for OA, respectively.The peak hemolymph concentration and the time to peak hemolymph concentration following oral dose were 21.1 μg/ml at 4 h for OTC and 4.20 μg/ml at 4 h for OA. The bioavailability after oral administration was 35.6% for OTC and 7.9% for OA. The elimination half-life of muscle was 22.0 h for OTC and 20.2 h for OA. The elimination half-life of shell was 21.9 h for OA, however, for OTC the elimination half-life of shell could not calculated because the drug residues persisted in shell tissues.Residual OTC in muscle was reduced to 50〜60% by the boiling for 4 min and frying at 180℃ for 1 min, and 30〜40% baking at 200℃ for 4 min. However, the reduction levels in shell were only 20%. Residual OA was 20〜30% in muscle and shell by the cooking.

研究了土霉素（OTC）和恶喹酸（OA）在斑节对虾（Penaeus monodon，18-25 g虾）体内的药代动力学和残留。将虾保存在具有盐度为5-10 ppt的海水的罐中。将水温调节至28-29℃。窦内给药后两种药物的血淋巴浓度最好用两室开放模型描述。获得以下方程来描述两种药物的血淋巴水平的时间过程：土霉素：Ct=35.4·exp（-3.5303统计）+10.6统计指数（-0.0429统计）恶喹酸：Ct=9.96·exp（-0.8252 statistt）+3.88 statistexp（-0.0391 statistt）土霉素的分布和消除半衰期分别为0.2和16.2 h，OA为0.84和17.7 h。OTC的稳态表观分布容积和全身清除率估计值分别为869 ml/kg和38.7 ml/kg/h，OA分别为2，061 ml/kg和90.1 ml/kg/h。口服给药后，OTC的血淋巴峰浓度和血淋巴峰浓度达峰时间分别为21.1 μg/ml（4 h）和4.20 μg/ml（4 h）。口服给药后，OTC和OA的生物利用度分别为35.6%和7.9%。肌肉消除半衰期为22.0 h（OTC）和20.2 h（OA）。甲壳中OA的消除半衰期为21.9 h，而OTC的消除半衰期因药物残留在甲壳组织中而无法计算，肌肉中OTC的残留量经水煮4 min、180℃油炸1 min和200℃烘烤4 min后可降至50 ~ 60%。壳中的减少水平仅为20%。经蒸煮处理后，肉和壳中OA残留量为20 ~ 30%。