Development of the gene deletion model rats by random mutagenesis.

通过随机诱变开发基因缺失模型大鼠。

• 批准号: 16500282
• 负责人: HARADA Yoshinobu
• 金额: $ 2.43万
• 依托单位: National Institute of Radiological Sciences (NIRS)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500282/
• 关键词: rat; chlorambucil; mutagenesis; gene deletion; model animal; 遺伝子欠損; モデル運動; マイクロアレイ

Random mutagenesis with chlorambucil was performed in order to produce some new mutant rats. To identify the optimal quantity of chlorambucil and the optimal mating time for mutagenesis, we counted the number of new born rats born from female rats mated with the male rat medicated with several dose of chlorambucil. Consequently, it became clear that between 2.5mg and 15mg per kg weight of chlorambucil are good for medication, and it is good to perform mating between three weeks and six weeks after the medication of chlorambucil. We obtained several dwarfish rats from BN and F344 strans. Dwarfish rats whose sizes of the body were about 30% of normal rats were also observed. We also obtained a mutant rat with fiblosarcoma from the BN strain. This fiblosarcoma has grown up to 5cm or more in the diameter within 2 weeks. Unfortunately, we have not determined the genome region of delition of this rat by quontitative PCR analysis. Future experiments will focus on searching this genome region of delition by microarray or BAC array analyses.

为了产生一些新的突变大鼠，用氯胺丁苯进行了随机诱变。为了确定最佳剂量的百菌清诱变交配时间和最佳诱变交配时间，我们统计了不同剂量的百菌清与雄性大鼠交配后所产仔鼠的数量。因此，很明显，每公斤重量2.5毫克到15毫克的氯氨丁腈适合服药，并且在服药后三周到六周之间进行交配是好的。我们从BN和F344链中获得了几只侏儒鼠。还观察了体重约为正常大鼠的30%的侏儒大鼠。我们还从BN株中获得了一只患有成纤维肉瘤的突变大鼠。成纤维肉瘤在2周内已生长到直径5厘米或更大。遗憾的是，我们还没有通过定量PCR分析来确定这只大鼠的基因组缺失区域。未来的实验将集中在通过微阵列或BAC阵列分析来寻找这一基因组缺失区域。

项目成果

Fractionated irradiation augments inter-strain variation of skin reactions among three strains of mice

• DOI: 10.1269/jrr.45.515
• 发表时间: 2004-12-01
• 期刊: JOURNAL OF RADIATION RESEARCH
• 影响因子: 2
• 作者: Ohta, T;Iwakawa, M;Imai, T
• 通讯作者: Imai, T

Radiation sensitivities of 31 human oesophageal squamous cell carcinoma cell lines

• DOI: 10.1111/j.0959-9673.2005.00431.x
• 发表时间: 2005-08-01
• 期刊: INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY
• 影响因子: 3
• 作者: Ban, S;Michikawa, Y;Imai, T
• 通讯作者: Imai, T

Improved in Vitro Fertilization and Development by Use of Modified Human Tubal Fluid and Applicability of Pronucleate Embryos for Cryopreservation by Rapid Freezing in Inbred Mice,

使用改良的人输卵管液改善体外受精和发育，以及通过快速冷冻冷冻保存原核胚胎在近交小鼠中的适用性，

• 发表时间: 2004
• 期刊: Comparative Medicine 54・5
• 作者: Kito;S
• 通讯作者: S

Radiosensitivity of peripheral blood lymphocytes obtained from patients with cancers of the breast, head & neck or cervix as determined with a micronucleus assay

乳腺癌、头颅癌患者外周血淋巴细胞的放射敏感性

• 发表时间: 2004
• 期刊: Journal of Radiation Research 45(4)
• 作者: Takai;N;Sun Xue Zhi;Toshie Oota;Sadayuki Ban
• 通讯作者: Sadayuki Ban

Experimental model for irradiating a restricted region of the rat brain using heavy-ion beams.

使用重离子束照射大鼠大脑受限区域的实验模型。

• 发表时间: 2004
• 期刊: The Journal of Medical Investigation 51・1,2
• 作者: Takai;N;Sun Xue Zhi
• 通讯作者: Sun Xue Zhi