Development of the gene deletion model rats by random mutagenesis.

用氯霉素进行随机诱变，以产生一些新的突变大鼠。为了确定氯霉素的最佳剂量和最佳交配时间，我们统计了给药不同剂量氯霉素的雄鼠与雌鼠交配的新生大鼠数量。因此，氯苯在每公斤体重2.5 ~ 15mg之间为宜用药，在氯苯用药后3 ~ 6周内进行交配为宜。我们从BN和F344株中获得了几只侏儒大鼠。体型约为正常大鼠30%的侏儒大鼠也被观察到。我们还从BN菌株中获得了一只纤维肉瘤突变大鼠。纤维肉瘤在两周内生长到直径5厘米或更大。不幸的是，我们没有通过定量PCR分析确定该大鼠缺失的基因组区域。未来的实验将集中于通过微阵列或BAC阵列分析来搜索缺失的基因组区域。

Random mutagenesis with chlorambucil was performed in order to produce some new mutant rats. To identify the optimal quantity of chlorambucil and the optimal mating time for mutagenesis, we counted the number of new born rats born from female rats mated with the male rat medicated with several dose of chlorambucil. Consequently, it became clear that between 2.5mg and 15mg per kg weight of chlorambucil are good for medication, and it is good to perform mating between three weeks and six weeks after the medication of chlorambucil. We obtained several dwarfish rats from BN and F344 strans. Dwarfish rats whose sizes of the body were about 30% of normal rats were also observed. We also obtained a mutant rat with fiblosarcoma from the BN strain. This fiblosarcoma has grown up to 5cm or more in the diameter within 2 weeks. Unfortunately, we have not determined the genome region of delition of this rat by quontitative PCR analysis. Future experiments will focus on searching this genome region of delition by microarray or BAC array analyses.