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Effects of indirubin a novel interval Ahreceptav ligand on the devepmeutard reproductive function of human fetus

新型间隔Ahreceptav配体靛玉红对胎儿生殖功能发育的影响

基本信息

批准号：
16510033
负责人：
TAKAI Yasushi
金额：
$ 2.18万
依托单位：
Saitama Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16510033/
关键词：
endocrine disrupting chemicals indirubin fetal exposure oocyte apoptosis ダイオキシン類

项目摘要

1. Maternal and fetal exposure to dioxinsThe concentrations of dioxins (PCDDs, PCDFs and Co-PCBs) were determined in maternal and umbilical cord blood and amniotic fluid. There was a significant positive correlation between maternal age and maternal blood Co-PCBs, and also a significant positive correlation in total dioxin, PCDDs and Co-PCBs concentration between the maternal and umbilical cord blood, suggesting transplacental dioxin translocation. It was also shown that PCDDs and PCDFs are likely to accumulate in the lipid components in the amniotic fluid. Moreover, it was suggested that fetal dioxin exposure may affect fetal and neonatal thyroid function.2. Effects of prenatal exposure to indirubin on offspringIndirubin, which was found in human urine, has 50-times higher affinity to dioxin receptor (Ah receptor) and its biological function remains to be elucidated. Pregnant mice were administered on 14.5 dpc with 1) indirubin, 2) 7,8-benzoflavone (Ah receptor inhibitor), 3) indirubi … More n + 7,8-benzoflavone, and 4) DMSO alone. Birth weight of pups in groups 1) and 2) were significantly lower in those in 3) and 4), and follicle numbers in the ovaries on postnatal day 4 was lower in indirubin-treated groups.3. Indirubin concentration in maternal urine and amniotic fluidIndirubin concentration in the maternal blood in first (12-15 weeks), second (21-24 weeks), and third (33-36 weeks) trimester of their gestational period was determined by indirubin-specific ELJSA. Indirubin level in third trimester was significantly higher than those in first trimester. Birth weight, however, was not significantly correlated with the maternal indirubin level. Indirubin level was below the detectable limits in the amniotic fluid obtained in second and third trimester.These results suggest that fetal exposure to indirubin may affect neonatal development and reproductive function, and that pregnant women are likely to be exposed to higher level of indirubin than non-pregnant women. However, it is also suggested that indirubin exposure of human fetus may remain insignificant. Less

1.母亲和胎儿暴露于二恶英二恶英（PCDDs，PCDFs和Co-PCBs）的浓度测定在母亲和脐带血和羊水。孕妇年龄与孕妇血中Co-PCBs浓度呈显著正相关，孕妇血和脐带血中二恶英、PCDDs和Co-PCBs浓度也呈显著正相关，提示二恶英经胎盘转运。研究还表明，多氯二苯并对二恶英和多氯二苯并呋喃很可能在羊水的脂质成分中积累。胎儿暴露于二恶英可能影响胎儿和新生儿甲状腺功能.靛玉红与二恶英受体（Ah受体）的亲和力比人尿中的高50倍，其生物学功能尚不清楚。孕14.5dpc时分别给予1）靛玉红，2）7，8-苯并吡喃酮（Ah受体抑制剂），3）靛玉红， ...更多信息 n +7，8-苯并二氢吡喃酮，和4）单独的DMSO。第1）和2）组的幼仔出生体重显著低于第3）和4）组，出生后第4天的卵巢卵泡数低于靛玉红处理组.采用靛玉红特异性ELJSA法测定妊娠早期（12-15周）、中期（21-24周）和晚期（33-36周）孕妇尿液和羊水中靛玉红浓度。孕晚期靛玉红水平明显高于孕早期。然而，出生体重与母体靛玉红水平无显著相关性。妊娠中期和妊娠晚期羊水中靛玉红水平均低于检测限，提示胎儿暴露于靛玉红可能影响新生儿发育和生殖功能，孕妇可能比非孕妇暴露于更高水平的靛玉红。然而，这也表明，靛玉红暴露的人胎儿可能仍然微不足道。少