Development of novel strategy for fertility preservation using cryopreserved ovarian tissue

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A fluorescence-activated cell sorting-based protocol was used with adult human ovarian cortical tissue to purify rare mitotically active cells that have a gene expression profile that is consistent with primitive germ cells. Once established in vitro, these cells (oogonial stem cells; OSCs) can be expanded for months and can spontaneously generate oocytes, as determined by morphology, gene expression and haploid (1n) status.Injection of the human OSCs into human ovarian cortical biopsies leads to formation of follicles containing oocytes 1-2 weeks after xenotransplantation into immunodeficient female mice. Thus, ovaries of reproductive-age women, similar to adult mice, possess OSCs that can be propagated in vitro as well as generate oocytes in vitro and in vivo.