Development of human ovarian tissue cryopreservation technique using ovaries derived from gender identity disorder patients

利用性别认同障碍患者的卵巢开发人类卵巢组织冷冻保存技术

• 批准号: 18591811
• 负责人: TAKAI Yasushi
• 金额: $ 2.64万
• 依托单位: Saitama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591811/
• 关键词: 卵巣; 凍結保存法; 性同一性障害; アポトーシス

Ovarian tissue cryopreservation and transplantation are becoming increasingly important issues for preserving fertility for young women with cancer as shown by recent successes in restoring ovarian activity and even fertility. Vitrification seems to be a promising technology in ovarian tissue cryopreservation, but it is still in an initial stage. Human ovarian tissue was obtained from consenting female-to-male GID (gender indentiry disorder) patients in sex reassignment surgery (SRS). Ovarian cortex tissues were cut into slices with the size of 1×10×10mm, which is suitable for transplantation in clinical situation, and cryopreserved by Cryotissue vitrification protocol. The frozen/thawed human ovarian tissues were xenografted into the ovarian bursa of non-obese diabetic severe combined immunodeficient (NOD-SCID) mice, and at least 1000 viable follicles were observed in each slice two weeks after transplantation.

卵巢组织冷冻保存和移植正成为年轻女性癌症患者保持生育能力的越来越重要的问题，最近在恢复卵巢活性甚至生育能力方面取得了成功。玻璃化冷冻是一种很有前途的卵巢组织冷冻保存技术，但目前仍处于起步阶段。人类卵巢组织从同意接受性别重置手术（SRS）的女性变男性GID（性别识别障碍）患者中获得。将卵巢皮质组织切成1×10× 10 mm的适合临床移植的切片，采用Cryotissue玻璃化冷冻法冷冻保存。将冷冻/解冻的人卵巢组织异种移植到非肥胖糖尿病严重联合免疫缺陷（NOD-SCID）小鼠的卵巢囊中，移植后2周，在每个切片中观察到至少1000个存活卵泡。

项目成果

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• 发表时间: 2006
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Elevated serum bisphenol A levels under hyperandrogenic conditions may be caused by decreased UDP-glucuronosyltransferase activity

• DOI: 10.1507/endocrj.k06-032
• 发表时间: 2006-08-01
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Association of human leukocyte antigen and T cell message with human papillomavirus 16–positive cervical neoplasia in Japanese women

• DOI: 10.1111/j.1525-1438.2007.00938.x
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• 发表时间: 2009
• 作者: Takai Y;Shinkai A;Hashimoto F;Asano T;Suzuki H;Kagawa N;Kuwayama M;Seki H;Takeda S;Ishihara O;高井泰;久保田 和子;高井泰
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• 发表时间: 2006
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