Utilization as Molecular Container of Layered Double Hydroxides for Vitamins

作为维生素层状双氢氧化物分子容器的用途

• 批准号: 16550164
• 负责人: NARITA Eiichi
• 金额: $ 2.37万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16550164/
• 关键词: Layered double hydroxide; Hydrotalcite; Vitamin C; Vitamin A acid; Intercalation; Vitamin stabilization; Deintercalation; Molecular container; 有機; 無機層状複合体; 安定化

For the development of biocompatible and health supporting materials, we have studied on the utilization of layered double hydroxide (LDH) as ‘Molecular container'. In this study, the intercalation of vitamin C (L-ascorbic acid) and vitamin A acid (retinoic acid) into tree kinds of LDH was investigated, as well as the stability of these guest vitamins in LDH and the release of vitamins from the resulting composites.First, Mg-Al,Mg-Fe and Zn-Al LDHs were adopteted as host hydroxide layers. It was found the vitamin C was intercalated only in Mg-Al and Mg-Fe LDHs by the reconstruction method and only in Mg-Al and Zn-Al LDHs by the coprecipitation method. The intercalation isotherms were obtained in each cases and all the data was well-treated by the Freundlich adsorption equation. Moreover, vitamin A acid was also intercalated in Mg-Al LDH.On the other hand, there is two types of vitamin C, i.e.,reduced type and oxidized type, and only the former demonstrates the reducing and physiological functions. After the white light irradiation treatment or heat treatment of the resulting vitamin/LDH composites, the amount of reduced type vitamin C in the composite was found to be about two times compared to that of the naked one in the same condition. Then, 82〜97% vitamin C was released from the composites by the anion exchange with carbonate ion. In addition, vitamin A acid was also stabilized in the interlayer of Mg-Al LDH and easily released by the same anion exchange.In conclusion, LDH can be used as ‘molecular container' for vitamins.

为了开发生物相容性和健康支持材料，我们研究了层状双氢氧化物(LDH)作为分子容器的应用。本文研究了维生素C(L抗坏血酸)和维生素A酸(维甲酸)在三种乳酸脱氢酶中的插层行为，以及这些客体维生素在乳酸脱氢酶中的稳定性和维生素的释放情况。结果表明，重构法只在镁铝和镁铁水滑石中嵌入维生素C，而共沉淀法只在镁铝和锌铝水滑石中嵌入维生素C。用Freundlich吸附方程对插层等温线进行了处理。此外，在镁铝低密度脂蛋白中还嵌入了维生素A酸。另一方面，维生素C有两种类型，即还原型和氧化型，只有前者具有还原和生理功能。经白光辐照处理或热处理后，在相同条件下，复合维生素C中还原型维生素C的含量约为裸露维生素C的两倍。然后，通过与碳酸盐离子的阴离子交换，从复合材料中释放出82~97%的维生素C。此外，维生素A酸也稳定在镁铝乳酸脱氢酶的层间，并容易被相同的阴离子交换释放，因此，乳酸脱氢酶可以作为维生素的分子容器。